Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-TrkA (Tyr490) Antibody #9141

NGF Receptor   NGFR   p140 (trk)   trk a   trkb   Trkl  

No. Size Price
9141L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9141S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9141 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 140 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-TrkA (Tyr490) Antibody detects endogenous levels of Trk only when phosphorylated at tyrosine 490. This antibody also detects TrkB and TrkC when phosphorylated at the corresponding residues.

Phospho-TrkA(Tyr490) Antibody 兔多抗识别内源性的Tyr490磷酸化的Trk蛋白。它也可以识别对应氨基酸残基磷酸化的TrkB和TrkC蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr490 of human TrkA. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类TrkA蛋白Tyr490位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or NGF-treated (100 ng/ml), using Phospho-TrkA (Tyr490) Antibody.

Western blot分析PC12细胞,未处理或经NGF(100 ng/ml)处理,使用的抗体是Phospho-TrkA (Tyr490)Antibody 兔多抗。

Background

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).

Trk受体酪氨酸激酶家族包括TrkA,TrkB和TrkC。这些家庭成员的序列是高度保守的,它们是由不同的神经营养因子激活:NGF激活TrkA,BDNF或NT4激活TrkB,NT3激活TrkC。TrkA调节细胞增殖,神经系统的发育和成熟很重要(1)。在Tyr490磷酸化,需要与Shc结合及Ras-MAP激酶级联的激活。Tyr674/675残基在催化域内,此位点的磷酸化能影响TrkA的激酶活性(2-6)。点突变,缺失和染色体重排(嵌合体)会引起配体依赖受体的二聚化,激活TrkA。许多恶性肿瘤,包括乳腺癌、结肠癌、前列腺癌、甲状腺癌和急性髓细胞白血病有激活的TrkA。神经母细胞瘤的TrkA表达是一个预后良好的指标,因为它标志着肿瘤生长停滞和从神经嵴分化而来(1)。

  1. Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
  2. Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89.
  3. Stephens, R.M. et al. (1994) Neuron 12, 691-705.
  4. Marsh, H.N. et al. (2003) J Cell Biol 163, 999-1010.
  5. Obermeier, A. et al. (1993) EMBO J 12, 933-41.
  6. Obermeier, A. et al. (1994) EMBO J 13, 1585-90.
  7. Arevalo, J.C. et al. (2001) Oncogene 20, 1229-34.
  8. Reuther, G.W. et al. (2000) Mol Cell Biol 20, 8655-66.
  9. Greco, A. et al. (1997) Genes Chromosomes Cancer 19, 112-23.
  10. Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8.
  11. Lagadec, C. et al. (2009) Oncogene 28, 1960-70.
  12. Greco, A. et al. (2010) Mol Cell Endocrinol 321, 44-9.
  13. Ødegaard, E. et al. (2007) Hum Pathol 38, 140-6.

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