Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3 (124H6) Mouse mAb #9139

sc-482   sc-8019  

No. Size Price
9139S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9139T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
9139 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 79, 86 Mouse IgG2a
IP 1:200
IHC-P 1:600
F 1:200
IF-IC 1:1600
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

Stat3 (124H6) Mouse mAb detects endogenous levels of total Stat3 protein.

Stat3 (124H6)鼠源抗体能够检测内源Stat3蛋白总体水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse Stat3.

此单克隆抗体是通过合成鼠源对应的Stat3蛋白序列的多肽来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Stat3 (124H6) Mouse mAb.Western免疫印迹。用Stat3 (124H6) Mouse mAb研究HeLa, NIH/3T3, PC12 和 COS细胞的细胞提取液。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left), showing nuclear and cytoplasmic staining, and human lung carcinoma (right), showing cytoplasmic staining, using Stat3 (124H6) Mouse mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织(左图),图片显示了细胞核和细胞质的染色和石蜡包埋人肺癌组织(右图),图片显示了细胞质的染色。所用抗体为Stat3 (124H6) Mouse mAb。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Hela cells, untreated (blue) or IFN-alpha-treated (green), using Stat3 (124H6) Mouse mAb compared with a nonspecific negative control antibody (red).流式细胞仪研究未经处理的HeLa细胞(蓝色)和经IFN-alpha处理的HeLa细胞(绿色)。所用抗体为Stat3 (124H6) Mouse mAb 和非特异性阴性对照抗体(红色)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells either serum-starved (left) or IFNalpha-treated (right) and labeled with Stat3 (124H6) Mouse mAb (green).共聚焦免疫荧光分析经血清饥饿的HeLa(左图)或者IFNalpha处理的(右图) HeLa,用Stat3 (124H6) Mouse mAb (绿色)标记。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 (124H6) Mouse mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。Hep G2 细胞培养至4 x 106, 并经过夜饥饿或IL-6(100 ng/ml,30 min) 处理,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用10 μl Stat3 (124H6) Mouse mAb 或2 μl Normal Rabbit IgG #2729 抗体。用人源 IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1),并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核和DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型,配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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