Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat3 (Ser727) (6E4) Mouse mAb #9136

Acute-phase response factor   APRF   pstat3   signal transducer and activator of transcription   stat3  

No. Size Price
9136S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9136 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 86 Mouse IgM

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Stat3 (Ser727) (6E4) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at serine 727. It does not significantly cross-react with the corresponding phosphorylated serines of other Stat proteins. The antibody does not cross-react with nonphosphorylated Stat3 or with Stat3 phosphorylated at other sites.

Phospho-Stat3 (Ser727) (6E4) Mouse mAb 只能检测内源的在Ser727位点磷酸的Stat3 蛋白。此抗体不与在对应丝氨酸残基磷酸化的其它Stat 蛋白交叉反应。此抗体也不与未磷酸化或者在别的位点磷酸化的Stat3蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser727 of mouse Stat3.

此单克隆抗体是通过合成鼠源对应的Stat3 Ser727位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved HeLa cells, untreated or IL-6-treated (100 ng/ml), using Phospho-Stat3 (Ser727) (6E4) Mouse mAb.Western免疫印迹。用Phospho-Stat3 (Ser727) (6E4) Mouse mAb研究未经处理的血清饥饿的HeLa细胞和经过IL-6 (100 ng/ml)处理的饥饿的HeLa细胞的细胞提取液。


The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1),并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核和DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型,配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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