Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3 Control Cell Extracts #9133

Acute-phase response factor   APRF   p-stat3   signal transducer and activator of transcription  

No. Size Price
9133S 100 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9133 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


Nonphosphorylated Stat3 Cell Extracts: Total cell extracts from serum-starved HeLa cells prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC. 
 Phosphorylated Stat3 Cell Extracts: Total cell extracts from serum-starved HeLa cells prepared with IFN-a treatment, serve as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.



CST recommends using 10 µl of phosphorylated and nonphosphorylated Stat3 extracts as controls.Note: These lysates are useful for Phospho-Stat3 (Tyr705) Antibody #9131, Phospho-Stat3 (Tyr705) (D3A7) #9145, Phospho-Stat3 (Tyr705) (3E2) #9138 and Phospho-Stat1 (Tyr701) Antibody #9171. However, they are not useful for Phospho-Stat3 (Ser727) Antibody #9134.

CST公司推荐使用10 µl Stat3磷酸化和未磷酸化的细胞提取液。注:此提取液适用于Phospho-Stat3 (Tyr705) Antibody #9131, Phospho-Stat3 (Tyr705) (D3A7) #9145, Phospho-Stat3 (Tyr705) (3E2) #9138 and Phospho-Stat1 (Tyr701) Antibody #9171但是不适用与Phospho-Stat3 (Ser727) Antibody #9134


The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1),并且被鼠科胚胎的发育所需要(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核和DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型,配体方向或者细胞发育的成熟阶段(10)。值得注意的是Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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