Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEK1 (Thr286) Antibody #9127

Mek1   mek1/2  

No. Size Price
9127S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9127 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 45 Rabbit
IP 1:50
F 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-MEK1 (Thr286) Antibody detects endogenous levels of MEK1 phosphorylated at threonine 286. This antibody does not cross-react with phosphorylated MEK2.

Phospho-MEK1 (Thr286) Antibody兔多抗能够检测内源性苏氨酸(286位点)磷酸化的MEK1蛋白水平。该抗体不会与磷酸化的MEK2蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human MEK1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A431, COS and PC12 cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody (upper) or MEK1 Antibody #9124 (lower).

western blot方法检测细胞提取物:未处理和诺考达唑处理的A431, COS和PC12细胞,使用的抗体是Phospho-MEK1 (Thr286) Antibody 兔多抗(上图)和MEK1 Antibody #9124 兔多抗(下图)。



Immunoprecipitation followed by Western blot analysis of extracts from COS cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody.

免疫沉淀实验之后用Western blot方法检测细胞提取物:未经处理的和诺考达唑处理的COS细胞,使用的抗体是Phospho-MEK1 (Thr286) Antibody兔多抗。



Confocal immunofluorescent analysis of mitotic HeLa cells labeled with Phospho-MEK1 (Thr286) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

激光共聚焦荧光法检测:标记Phospho-MEK1 (Thr286) Antibody (绿色)的HeLa细胞。肌动蛋白纤维素已被 Alexa Fluor® 555 phalloidin (红色)标记。蓝色伪彩为DNA荧光染料(产品信息为 DRAQ5®#4084 )。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-MEK1 (Thr286) Antibody versus propidium iodide (DNA content). The box indicates Phosho-MEK1 positive cells.

流式方法检测细胞提取物:未处理的Jurkat细胞,使用的抗体是Phospho-MEK1 (Thr286) Antibody兔多抗,对比碘化丙啶 (DNA含量)。


MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

MEK1、MEK2,也被称为MAPK或Erk激酶,它们是双特异性蛋白激酶,在丝裂原活化蛋白激酶级联反应中发挥作用,控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸(217 /221位点)的磷酸化而实现的,这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化,激活p44、 p42 MAPK激酶。

MEK1 is phosphorylated at Ser298 by PAK1, which facilitates signal transduction from Raf to MEK1 and Erk2 (5-7). MEK1 is also phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase pathway (8).

MEK1的Ser298位点被PAK1磷酸化后,能够促进信号从Raf 到 MEK1、Erk2的转导(5-7)。在处于有丝分裂的细胞中,MEK1的Thr286位点也会被cdk5磷酸化,导致p44/42 MAP激酶途径的负反馈 (8)。

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.
  5. Xu, B. et al. (1999) J. Biol. Chem. 274, 34029-34035.
  6. Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-2244.
  7. Eblen, S. T. et al. (2002) Mol. Cell. Biol. 22, 6023-6033.
  8. Sharma, P. et al. (2002) J. Biol. Chem. 277, 528-534.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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