Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEK1/2 (Ser217/221) Antibody #9121


No. Size Price
9121L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9121S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9121 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,S. cerevisiae, Endogenous 45 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

Phospho-MEK1/2 (Ser217/221) Antibody detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. This antibody does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221.

Phospho-MEK1/2 (Ser217/221) Antibody兔多抗能够检测内源性的Ser217/221位点磷酸化的MEK1/2蛋白水平。该抗体不会与其他相关激酶包括活化的SEK (MKK4), MKK3、MKK6发生交叉反应。它也能与Ser217位点和Ser221位点单独磷酸化的MEK1/2蛋白发生反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2. Antibodies are purified by protein A and peptide affinity chromatography.

该单克隆抗体是采用合成的与人源 MEK1/2蛋白Ser217/221位点周围相一致的磷酸化肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated, TPA-treated or lambda phosphatase-treated HeLa cells, using Phospho-MEK 1/2 (Ser217/221) Antibody #9121 (upper) or MEK 1/2 Antibody #9122 (lower).

使用Phospho-MEK 1/2 (Ser217/221) Antibody兔多抗 #9121 (上图)和MEK 1/2 Antibody兔多抗 #9122 (下图),对未处理和TPA处理、lambda磷酸酶处理的的HeLa细胞的提取物进行Western blot检测。


MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

MEK1、MEK2,也被称为MAPK或Erk激酶,它们是双特异性蛋白激酶,在丝裂原活化蛋白激酶级联反应中发挥作用,控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸(217 /221位点)的磷酸化而实现的,这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化,激活p44、 p42 MAPK激酶。

CST's Phospho- MEK1/2 (Ser217/221) Antibody selectively recognizes active MEK, i.e., only when phosphorylated at Ser217/221, and hence is an excellent marker of MEK1/2 activity.

CST的 Phospho- MEK1/2 (Ser217/221) Antibody兔多抗能选择性的识别活化的的MEK,即Ser217/221位点磷酸化的MEK,因此它是表征MEK1/2活性的重要标志。

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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