Cell Signaling Technology

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Pannexin-1 (D9M1C) Rabbit mAb #91137

sc-49695  

No. Size Price
91137S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
91137 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 45-55, 19 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Pannexin-1 (D9M1C) Rabbit mAb recognizes endogenous levels of total pannexin-1 protein. This antibody detects an amino-terminal pannexin-1 fragment produced by caspase cleavage.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human pannexin-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pannexin-1 (D9M1C) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, overnight; +), using Pannexin-1 (D9M1C) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human pannexin-1 protein (hPANX1; +), using Pannexin-1 (D9M1C) Rabbit mAb.

IP

IP

Immunoprecipitation of pannexin-1 from PANC-1 cell extracts. Lane is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Pannexin-1 (D9M1C) Rabbit mAb. Western blot was performed using Pannexin-1 (D9M1C) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG heavy chain.

Background

The pannexin family (pannexin-1, -2, and -3; PANX1-3) of gap junction proteins has homology to the invertebrate innexins and display distinct expression patterns (1). Pannexin-1 is widely expressed, with highest expression in the heart, brain, skeletal muscle, testis, and ovary (1,2). Pannexin-2 is predominately expressed in the brain (1,2) and pannexin-3 is found within the skin and connective tissues (1,3). Connexin family gap junction proteins form hemichannels that align adjacent cells, creating functional intercellular channels that are permeable to ions and small molecules. In contrast, pannexin proteins may not function as gap junction proteins since pannexins on adjacent cells may not align to form complete channels (3). These pannexin “hemichannels” may play a role in inflammation, apoptosis, and neuronal signaling by allowing permeability of ions, ATP, and potentially other small molecules into the extracellular space (4-6). Pannexin-1 can be activated by effector caspases (caspase-3 and -7), which leads to release of signal molecules that promote phagocytosis of apoptotic cells (7).

  1. Baranova, A. et al. (2004) Genomics 83, 706-16.
  2. Bruzzone, R. et al. (2003) Proc Natl Acad Sci U S A 100, 13644-9.
  3. Penuela, S. et al. (2007) J Cell Sci 120, 3772-83.
  4. Qu, Y. et al. (2011) J Immunol 186, 6553-61.
  5. Silverman, W.R. et al. (2009) J Biol Chem 284, 18143-51.
  6. MacVicar, B.A. and Thompson, R.J. (2010) Trends Neurosci 33, 93-102.
  7. Chekeni, F.B. et al. (2010) Nature 467, 863-7.

Application References

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