Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc2 (Tyr15) Antibody #9111

cdc2   CDC28   cdk1   MPF   p34cdc2  

No. Size Price
9111L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9111S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9111T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9111 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,Xenopus,S. cerevisiae, Endogenous 34 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-cdc2 (Tyr15) Antibody detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15. The antibody does not cross-react with CDK4, CDK6 or CDK7. It does detect the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19. Phospho-cdc2 (Tyr15) Antibody 能够检测内源性酪氨酸(15位)磷酸化的cdc2, CDK2 和CDK5蛋白。该抗体不与CDK4, CDK6或CDK7发生交叉反应,但能够识别酪氨酸19位磷酸化cdc2 (cdc28)蛋白的酵母同源序列。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对cdc2蛋白酪氨酸(15位)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from Saos cells, either untreated or treated with hydroxyurea or nocodazole, using Phospho-cdc2 (Tyr15) Antibody #9111 (upper) or cdc2 Antibody #9112 (lower). western blot方法检测未处理和羟基脲或 nocodazole处理的Saos细胞提取物,使用的抗体为Phospho-cdc2 (Tyr15) Antibody #9111 (上图) 或cdc2 Antibody #9112 (下图)。

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂,cdc2的激活受多个步骤的调控,包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而,在细胞进入有丝分裂的过程中,激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位,抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。

  1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
  2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
  3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
  4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
  5. Hunter, T. (1995) Cell 80, 225-36.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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