Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106

ERK   erk 1/2   ERK1   ERK2   mapk   p42   p44   p44/42   p44/p42 MAPK   pmapk   sc-7383  

No. Size Price
9106L 600 µl ( 120 western blots ) ¥8,792.00 现货查询 购买询价
9106S 200 µl ( 40 western blots ) ¥3,900.00 现货查询 购买询价
9106 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Hamster,Monkey,Mink,D. melanogaster,Zebrafish,Bovine,Pig, Endogenous 42, 44 Mouse IgG1
F 1:2000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb鼠单抗能够检测内源性的Thr202和Tyr204被双重磷酸化的Erk1(Thr185和 Tyr187双重磷酸化的Erk2),以及Tyr204被单磷酸化后的p44和p42 MAPK(Erk1和Erk2)蛋白。该抗体与JNK/SAPK或p38 MAPK中的相应磷酸化残基没有交叉反应。免疫荧光检测中发现该抗体能与某些细胞系中的未知骨架蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.

该单克隆抗体是采用合成的与人源p44 MAPK的Thr202/Tyr204位点周围序列相一致的磷酸化肽段免疫动物而产生的。

Western Blotting

Western Blotting

Western blot analysis of extracts from FGF treated SK-N-MC cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (upper) or control p44/42 MAPK (Erk1/2) Antibody #9102 (lower).western blot方法检测细胞提取物:FGF处理的K-N-MC细胞,使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (u上图)和control p44/42 MAPK (Erk1/2) Antibody #9102 (下图)。

Western Blotting

Western Blotting

Specificity and sensitivity of the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb: This antibody reacts specifically with as little as 50 pg of phosphorylated MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated MAP kinase by Western blotting.Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb特异性敏感性分析:Western blotting分析发现该抗体能与仅仅50 pg的磷酸化MAP激酶发生特异性反应,但是不能与大于4 µg的非磷酸化MAP激酶发生反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (red) or PMA-treated (blue) ; and untreated (green) or PMA-treated (purple) following 90-minute CIP treatment, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb.Flow cytometric方法检测细胞提取物:未经处理的(红色)、PMA处理的(蓝色),以及未经处理的(绿色)、PMA(紫色)+90min CIP处理的Jurkat细胞,使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb。


Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

丝裂原活化蛋白激酶(MAPKs)是一个具有广泛保守性的丝氨酸/苏氨酸蛋白激酶家族,在许多细胞过程中起作用,比如细胞增殖、分化、运动和死亡。p44/42 MAPK (Erk1/2)信号转导通路能在应答各种胞外刺激后被激活,这些刺激因子包括有丝分裂原、生长因子和细胞因子(1-3),并且被研究者认为是诊断和治疗癌症的重要靶点(4)。受到刺激后,三个不同层次的蛋白激酶级联反应就连续被开启,这三个层次的分子包含MAPKKK(MAP3K)、MAPKK(MAP2K)、MAPK。多种p44/42 MAP3Ks已经被鉴定出来,包括Raf家族成员,以及Mos、Tpl2/Cot。MEK1、MEK2是该通路中主要的MAPKKs(5,6)。MEK1、MEK2通过分别磷酸化p44和p42蛋白的活化环内的Thr202/Tyr204位点和Thr185位点/Tyr187位点,从而激活p44、p42。目前已经鉴定出p44/42的一些下游靶点,包括p90RSK (7) 和下游转录因子Elk-1 (8,9)。双特异性(Thr/Tyr) MAPK磷酸酶家族成员DUSPs、MKPs (10),以及MEK抑制子如U0126、 PD98059,可以负调节p44/42。

  1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
  2. Baccarini, M. (2005) FEBS Lett 579, 3271-7.
  3. Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
  4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
  5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
  6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
  7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  8. Marais, R. et al. (1993) Cell 73, 381-93.
  9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
  10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
  11. Suzuki, M. et al. (2015) J Immunol 195, 1273-81.

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