Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) #9072

No. Size Price
9072S 100 µl ( 10 immunoprecipitations ) ¥3,900.00 现货查询 购买询价
9072 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:50 Human, Endogenous Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Chicken, Xenopus, Zebrafish, Bovine, Pig, Horse,

Specificity / Sensitivity

Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) recognizes endogenous levels of histone H2B protein only when acetylated at Lys12. This antibody does not cross-react with histone H2B acetylated at Lys5, Lys15, or Lys20.

Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb 兔单抗(ChIP Formulated)能够检测仅在Lys12位点乙酰化的内源性histone H2B蛋白。该抗体不能与Lys5、Lys15或Lys20位点乙酰化的histone H2B发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys12 of human histone H2B protein.

该单克隆抗体是采用合成的与人源histone H2B蛋白Lys12位点周围相应的乙酰化的片段免疫动物而成产的。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 µl of Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,用4 x 106 HeLa细胞的交联染色质以及10 µl Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用SimpleChIP® Human GAPDH Exon 1 Primers #5516、SimpleChIP® Human RPL30 Exon 3 Primers #7014、SimpleChIP® Human MyoD1 Exon 1 Primers #4490和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于1的总input chromatin的数量的信号。



Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated) specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated acetyl-histone H2B (Lys12) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the acetyl-histone H2B (Lys12) peptide competed away binding of the antibody.

通过peptide ELISA确定Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)的特异性。该图描述了抗体与提前包被的acetyl-histone H2B (Lys12) peptide的结合能力,并且多肽中含有浓度递增的不同竞争多肽。如同所示,仅acetyl-histone H2B (Lys12) peptide竞争脱离抗体的结合。


The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). The p300/CBP histone acetyltransferases acetylate multiple lysine residues in the amino terminal tail of histone H2B (Lys5, 12, 15, and 20) at gene promoters during transcriptional activation (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the access of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites that facilitate recruitment of many transcription and chromatin regulatory proteins that contain a bromodomain, which binds to acetylated lysine residues (6). Histone H2B is mono-ubiquitinated at Lys120 during transcriptional activation by the RAD6 E2 protein in conjunction with the BRE1A/BRE1B E3 ligase (also known as RNF20/RNF40) (7). Mono-ubiquitinated histone H2B Lys120 is associated with the transcribed region of active genes and stimulates transcriptional elongation by facilitating FACT-dependent chromatin remodeling (7-9). In addition, it is essential for subsequent methylation of histone H3 Lys4 and Lys79, two additional histone modifications that regulate transcriptional initiation and elongation (10). In response to metabolic stress, AMPK is recruited to responsive genes and phosphorylates histone H2B at Lys36, both at promoters and in transcribed regions of genes, and may regulate transcriptional elongation (11). In response to multiple apoptotic stimuli, histone H2B is phosphorylated at Ser14 by the Mst1 kinase (12). Upon induction of apoptosis, Mst1 is cleaved and activated by caspase-3, leading to global phosphorylation of histone H2B during chromatin condensation. Interestingly, histone H2B is rapidly phosphorylated at irradiation-induced DNA damage foci in mouse embryonic fibroblasts (13). In this case, phosphorylation at Ser14 is rapid, depends on prior phosphorylation of H2AX Ser139, and occurs in the absence of apoptosis, suggesting that Ser14 phosphorylation may have distinct roles in DNA-damage repair and apoptosis.

核小体是由四种中心组蛋白(H2A、H2B、H3和H4)组成,它是染色质的主要构件模块。起初被认为是一个DNA包装的静态支架,目前组蛋白已经被认为是一个动态蛋白,其经历多种形式的翻译后修饰,包括乙酰化作用、磷酸化作用、甲基化作用和泛素化作用(1,2)。在转录激活期间,p300/CBP组蛋白乙酰化转移酶在histone H2B (Lys5、12、15和20)基因启动子的氨基端使多个赖氨酸残基乙酰化 (1-3)。组蛋白尾部的高度乙酰化中和了这些区域的正电荷,并且被认为弱化了组蛋白-DNA和核小体-核小体的相互作用,因此使核染色质不稳定以及增加了DNA与不同的DNA结合蛋白的接近程度(4,5)。此外,特定的赖氨酸残基乙酰化产生了停留点,这些位置有助于招募许多转录和染色质调节蛋白,这些蛋白包含一个bromodomain,该区域能结合到乙酰化的赖氨酸残基(6)。在转录激活期间,通过RAD6 E2蛋白连接BRE1A/BRE1B E3 ligase (also known as RNF20/RNF40)从而使Histone H2B在Lys120位点发生单泛素化(7)。单泛素化的histone H2B Lys120是与活化基因的转录区域有关联,并且通过促进FACT依赖的染色质重构从而刺激转录延伸(7-9)。此外,对于随后的histone H3在Lys4 和Lys79位点的甲基化有本质作用,这两个附加的组蛋白修饰能调节转录起始和延伸(10)。在代谢应激的反应下,AMPK被招募到反应性基因以及使histone H2B在Lys36位点磷酸化,这都发生在基因的启动子和转录区域,以及这些可能调节转录的延伸(11)。在多种凋亡刺激下,histone H2B通过Mst1激酶使其Ser14位点磷酸化(12)。在凋亡的诱导下,Mst1激酶被剪切,并且通过caspase-3激活,导致在染色质浓缩期间histone H2B整体的磷酸化。有趣的是,在小鼠胚胎成纤维细胞中histone H2B在照射诱导DNA损伤点发生快速磷酸化(13)。既然这样,在Ser14位点的磷酸化是快速的,这取决于在H2AX Ser139位点的优先磷酸化,并且是发生在没有凋亡情况下,从而认为Ser14位点磷酸化可能在DNA损伤的修复和凋亡中有明显的作用。

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  2. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
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  5. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
  6. Yang, X.J. (2004) Bioessays 26, 1076-87.
  7. Kim, J. et al. (2009) Cell 137, 459-71.
  8. Minsky, N. et al. (2008) Nat Cell Biol 10, 483-8.
  9. Pavri, R. et al. (2006) Cell 125, 703-17.
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  11. Bungard, D. et al. (2010) Science 329, 1201-5.
  12. Cheung, W.L. et al. (2003) Cell 113, 507-17.
  13. Fernandez-Capetillo, O. et al. (2004) J Exp Med 199, 1671-7.

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