Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RBAP46/RBAP48 (D4F8) Rabbit mAb #9067

No. Size Price
9067S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
9067 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 48 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Hamster, Chicken, Guinea Pig,

Specificity / Sensitivity

RBAP46/RBAP48 (D4F8) Rabbit mAb recognizes endogenous levels of total RBAP46 and RBAP48 proteins.

RBAP46/RBAP48 (D4F8) Rabbit mAb兔单抗检测内源性RBAP46和RBAP48总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RBAP48 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBAP46/RBAP48 (D4F8) Rabbit mAb.

使用RBAP46/RBAP48 (D4F8) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中RBAP46/RBAP48蛋白水平。


Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAP46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).

Retinoblastoma-associated proteins 46和48 (RBAP46和RBAP48;又称为RBBP7和RBBP4)在人源细胞中首先以结合到retinoblastoma (Rb)肿瘤抑制蛋白为特征(1)。从那时以来,这些蛋白质已经显示是许多蛋白质复合物中的成分,这些复合物涉及染色质调节,包括chromatin assembly factor 1 (CAF-1)复合物和type B组蛋白乙酰转移酶复合物HAT1,在DNA复制期间这两个复合物的功能是染色质的装配(2,3)。RBAP46和RBAP48蛋白也被发现在核小体重构因子复合物NURF、核小体重塑和组蛋白去乙酰化复合物NuRD和Sin3/HDAC组蛋白去乙酰化复合物(4-7)。最近,RBAB46和RBAP48被鉴定作为polycomb repressor complex PRC2的成分,该复合物也包含EED和Ezh2 (8)。RBAP46和RBAP48结合到组蛋白histone H4折叠区域,以及认为有助于这些染色质重塑、组蛋白乙酰化和组蛋白去乙酰化复合物作用于它们的组蛋白底物上(3)。

  1. Qian, Y.W. et al. (1993) Nature 364, 648-52.
  2. Verreault, A. et al. (1996) Cell 87, 95-104.
  3. Verreault, A. et al. (1998) Curr Biol 8, 96-108.
  4. Barak, O. et al. (2003) EMBO J 22, 6089-100.
  5. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
  6. Zhang, Y. et al. (1997) Cell 89, 357-64.
  7. Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905.
  8. Cao, R. et al. (2002) Science 298, 1039-43.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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