Cell Signaling Technology

Product Pathways - Innate Immunity

Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate) #90662

CD207   Langerin  

No. Size Price
90662S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
90662 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Langerin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val14 of human langerin protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Langerin (D9H7R) XP® Rabbit mAb #13650.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MUTZ-3 cells, undifferentiated (blue) or differentiated (green) into Langerhans-like cells by treatment with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (10ng/ml), Human Transforming Growth Factor β1 (hTGF-β1) #8915 (10ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5ng/ml) for 10 days, using Langerin (D9H7R) XP® Rabbit mAb (PE Conjugate).

Background

Langerin (CD207) is a C-type lectin receptor whose expression is restricted mainly to dendritic cells in the skin including Langerhans cells in the epidermis and langerin+/CD103+ dermal dendritic cells (1-4). Langerin is found on the cell surface and within rod-shaped organelles called Birbeck granules, and its expression is required for the formation of Birbeck granules (1,5). Langerin recognizes carbohydrate motifs on the surface of pathogens, resulting in endocytosis of the pathogen into Birbeck granules, degradation of the pathogen, and antigen presentation to T cells (6-8).

  1. Valladeau, J. et al. (2000) Immunity 12, 71-81.
  2. Poulin, L.F. et al. (2007) J Exp Med 204, 3119-31.
  3. Ginhoux, F. et al. (2007) J Exp Med 204, 3133-46.
  4. Bursch, L.S. et al. (2007) J Exp Med 204, 3147-56.
  5. Kissenpfennig, A. et al. (2005) Mol Cell Biol 25, 88-99.
  6. Stambach, N.S. and Taylor, M.E. (2003) Glycobiology 13, 401-10.
  7. Idoyaga, J. et al. (2008) J Immunol 180, 3647-50.
  8. Igyártó, B.Z. et al. (2011) Immunity 35, 260-72.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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