Cell Signaling Technology

Product Pathways - Metabolism

MMS19 (D5J8J) Rabbit mAb #90637

No. Size Price
90637S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
90637 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 100 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MMS19 (D5J8J) Rabbit mAb recognizes endogenous levels of total MMS19 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu892 of human MMS19 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MMS19 (D5J8J) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human MMS19 (hMMS19-Myc/DDK; +), using MMS19 (D5J8J) Rabbit mAb.

Background

Iron-sulfur (Fe-S) clusters (ISC) are cofactors for many proteins that display a wide range of biological functions, such as DNA maintenance, transcription, translation, cellular metabolism, electron transport, and oxidative phosphorylation (1). While structurally simple, the synthesis and insertion of ISC into Fe-S proteins are complex processes that involve many different proteins. MMS19 (methyl-methanesulfonate sensitivity 19) is a key component of the cytosolic Fe-S protein assembly complex, acting as an adaptor for a subset of Fe-S proteins including XPD and FANCJ, therefore playing an important role in DNA repair and transcription (2-4). MMS19 may also function as a coactivator for the estrogen receptor (5). In addition it has been reported that MMS19 is in a complex that is localized to the mitotic spindle and is required for proper chromosome segregation during mitosis (6).

  1. Wang, J. and Pantopoulos, K. (2011) Biochem J 434, 365-81.
  2. Stehling, O. et al. (2012) Science 337, 195-9.
  3. Gari, K. et al. (2012) Science 337, 243-5.
  4. Vashisht, A.A. et al. (2015) J Biol Chem 290, 14218-25.
  5. Wu, X. et al. (2001) J Biol Chem 276, 23962-8.
  6. Ito, S. et al. (2010) Mol Cell 39, 632-40.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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