Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

PIAS3 (D5F9) XP® Rabbit mAb #9042

No. Size Price
9042S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
9042T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
9042 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 65-75 Rabbit IgG
IP 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

PIAS3 (D5F9) XP® Rabbit mAb recognizes endogenous levels of total PIAS3 protein.

PIAS3 (D5F9) XP® Rabbit mAb兔单抗能够检测内源性PIAS3总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro590 of human PIAS3 protein.

此单克隆抗体通过合成肽免疫动物制备,这种合成肽是人源PIAS3蛋白Pro590周围的肽段。

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-204 (positive, left) and Hep G2 (low expression, right) cells using PIAS3 (D5F9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).共聚焦分析A-204(阳性,左图)和HepG2(低表达,右图)细胞,使用抗体是PIAS3 (D5F9) XP® Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin标记(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PIAS3 (D5F9) XP® Rabbit mAb. Western blot分析多种细胞系的细胞提取物,使用抗体是PIAS3 (D5F9) XP® Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human PIAS3 (hPIAS3; +), using PIAS3 (D5F9) XP® Rabbit mAb. Western blot分析293T细胞的细胞提取物,对照转染(-)或转染表达全长人源PIAS3(hPIAS3; +)的构建质粒,使用抗体是 PIAS3 (D5F9) XP® Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PIAS3 siRNA I #9073 (+), or SignalSilence® PIAS3 siRNA II #9031 (+), using PIAS3 (D5F9) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PIAS3 (D5F9) XP® Rabbit mAb confirms silencing of PIAS3 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot分析RD细胞的细胞提取物,转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PIAS3 siRNA I #9073 (+), 或 SignalSilence® PIAS3 siRNA II #9031 (+),使用抗体是PIAS3 (D5F9) XP® Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。PIAS3 (D5F9) XP® Rabbit mAb证实了PIAS3表达的沉默,β-Actin (D6A8) Rabbit mAb用来检测内参。

Background

The protein inhibitor of activated Stat (PIAS) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors (1,2). PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively (1-3). Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection (4). The PIAS family contains a conserved RING domain that has been linked to a function as a small ubiquitin-related modifier (SUMO) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins (5,6). Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (10), and Smads (11,12). The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity (13). In addition, PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1 (14).

PIAS (激活的Stat蛋白抑制剂)蛋白, 包括PIAS1, PIAS3, PIASx 和 PIASy,他们最初是通过他们与Stat转录因子的相互作用被鉴定(1,2)。PIAS1, PIAS3 和 PIASx分别与Stat1, Stat3 和 Stat4相互作用并抑制他们(1-3)。PIAS1的缺失导致了对干扰素诱导的基因的抑制并增强了对感染的抵抗(4)。PIAS家族的成员包含一个保守的RING区域,此区域的功能类似于 SUMO (小泛素相关修饰)连接酶, 偶联SUMO结合酶Ubc9及其底物蛋白(5,6)。大量的研究表明PIAS家族的成员能够通过不同的机制调控转录因子的活性,包括NF-κB (7,8)、c-Jun、p53 (5,9)、Oct-4 (11)和 Smads (11,12)。PIAS1的活性是通过磷酸化和精氨酸甲基化调节的。炎症刺激能够诱导IKK介导PIAS1在Ser90位点的磷酸化,这对其激活是必需的(13)。另外,在干扰素的处理下PRMT1诱导PIAS1在Arg303处的甲基化,这与其抑制Stat1活性相关(14)。

  1. Liu, B. et al. (1998) Proc Natl Acad Sci USA 95, 10626-31.
  2. Chung, C.D. et al. (1997) Science 278, 1803-5.
  3. Arora, T. et al. (2003) J Biol Chem 278, 21327-30.
  4. Liu, B. et al. (2004) Nat Immunol 5, 891-8.
  5. Schmidt, D. and Müller, S. (2002) Proc Natl Acad Sci USA 99, 2872-7.
  6. Kotaja, N. et al. (2002) Mol Cell Biol 22, 5222-34.
  7. Liu, B. et al. (2005) Mol Cell Biol 25, 1113-23.
  8. Tahk, S. et al. (2007) Proc Natl Acad Sci USA 104, 11643-8.
  9. Bischof, O. et al. (2006) Mol Cell 22, 783-94.
  10. Tolkunova, E. et al. (2007) J Mol Biol 374, 1200-12.
  11. Long, J. et al. (2004) Proc Natl Acad Sci USA 101, 99-104.
  12. Murdoch, R.N. and Edwards, T. (1992) Biochem Int 28, 1029-37.
  13. Liu, B. et al. (2007) Cell 129, 903-14.
  14. Weber, S. et al. (2009) Genes Dev 23, 118-32.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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