Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Ikaros (D10E5) Rabbit mAb #9034


No. Size Price
9034S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9034 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 50-70 Rabbit IgG
F 1:200
IF-IC 1:200
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

Ikaros (D10E5) Rabbit mAb recognizes endogenous levels of total Ikaros protein.

Ikaros (D10E5) Rabbit mAb检测内源性Ikaros总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg439 of human Ikaros protein.




Confocal immunofluorescent analysis of Jurkat cells (positive; left) and HeLa cells (negative; right) using Ikaros (D10E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 对Jurkat细胞(阳性;左图)和HeLa细胞(阴性;右图)进行共聚焦分析,使用抗体是Ikaros (D10E5) Rabbit mAb (绿色)。 肌动蛋白微丝使用DY-554 phalloidin 标记(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ikaros (D10E5) Rabbit mAb (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). Western blot results confirm absence of detectable Ikaros protein in HeLa cells, in accordance with the immunofluorescence analysis.Western blot分析多种细胞系的细胞提取物,使用抗体是Ikaros (D10E5) Rabbit mAb (上图)或β-actin (D6A8) Rabbit mAb #8457 (下图)。Western blot结果证明了HeLa细胞中未检测到Ikaros,这与免疫荧光分析结果相一致。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells using Ikaros (D10E5) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.对Ramos细胞进行流式细胞术分析,使用抗体是Ikaros (D10E5) Rabbit mAb (蓝色),concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412用来作为二抗。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Jurkat cells and either 10 μl of Ikaros (D10E5) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Ikaros family proteins, characterized by the presence of an N-terminal zinc finger DNA-binding domain and a C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. The Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers and heterodimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2). 
 Ikaros, also known as Ikaros family zinc finger protein 1 (IKZF1) and Lymphoid transcription factor 1 (LYF-1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Research studies have shown that imbalanced expression of different Ikaros isoforms, as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7).

Ikaros家族蛋白属于Kruppel转录因子超家族,其特点是在N-末端存在锌指DNA-结合域,在C-末端存在二聚化区域。Ikaros家族包括Ikaros、Aiolos、Helios,很可能还有EOS 和Pegasus (1)。他们能形成同源二聚体,也可以与其它Ikaros成员形成异源二聚体。由于存在不同的剪切而形成多种形式,有些家族成员在二聚化的作用下显现出显性失活现象(2)。Ikaros也叫做IKZF1 (Ikaros家族锌指蛋白1) 和LYF-1 (淋巴转录因子1),它主要是在血细胞中高表达 。在小鼠中,遗传学研究表明Ikaros对正常的T细胞、B细胞、NK细胞和树突状细胞的发育非常重要,并且也作为肿瘤抑制因子(3,4)。Ikaros不同形式表达的不平衡性以及不完整Ikaros的表达与人类的恶性血液病有关(5-7)。

  1. Perdomo, J. et al. (2000) J Biol Chem 275, 38347-54.
  2. Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
  3. Georgopoulos, K. et al. (1997) Annu Rev Immunol 15, 155-76.
  4. Nichogiannopoulou, A. et al. (1998) Semin Immunol 10, 119-25.
  5. Tonnelle, C. et al. (2002) Leuk Lymphoma 43, 29-35.
  6. Martinelli, G. et al. (2009) J Clin Oncol 27, 5202-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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