Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SPT5 Antibody #9033

No. Size Price
9033S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9033 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Guinea Pig, Horse,

Specificity / Sensitivity

SPT5 Antibody recognizes endogenous levels of total SPT5 protein.

SPT5 Antibody能够检测内源性SPT5总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly803 of human SPT5 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SPT5 Antibody.

使用SPT5 Antibody,免疫印迹(Western blot)分析不同细胞中SPT5的蛋白水平。


DRB-sensitivity inducing factor (DSIF), a heterodimer composed of SPT4 and SPT5, is capable of both facilitating and inhibiting RNA polymerase II (RNAPII) activity (1-3). DSIF, together with NELF (Negative Elongation Factor), inhibits RNAPII elongation, resulting in promoter proximal pausing of RNAPII as it awaits additional signaling to resume transcription (4). The release of promoter proximal pausing is signaled through phosphorylation of the RNAPII C-terminal domain (CTD) and NELF by positive transcription elongation factor (P-TEFb) (5). P-TEFb also phosphorylates SPT5 at Thr4 within the evolutionarily conserved heptapeptide repeat motif. This phosphorylation event switches DSIF from a transcriptional repressor to an activator where it becomes a critical factor for transcriptional elongation (6,7).

DRB-sensitivity inducing factor (DSIF)是有SPT4和SPT5组成的异源二聚体,它有能力去帮助和抑制RNA polymerase II (RNAPII)活性(1-3)。DSIF与NELF (Negative Elongation Factor)一起抑制RNAPII延伸,这导致RNAPII蛋白启动子近端暂停(proximal pausing),因为它等待别的信号去重新转录(4)。启动子近端暂停(proximal pausing)的释放是通过positive transcription elongation factor (P-TEFb)使RNAPII C-terminal domain (CTD)和NELF的磷酸化而发出的 (5)。 P-TEFb也使SPT5蛋白Thr4位点磷酸化,该位点存在于进化上保守的heptapeptide repeat结构域中。该磷酸化过程可以转变DSIF从一个转录抑制因子到一个激活因子,而激活形式对于转录延伸起着重要作用(6,7)。

  1. Wada, T. et al. (1998) Genes Dev 12, 343-56.
  2. Saunders, A. et al. (2006) Nat Rev Mol Cell Biol 7, 557-67.
  3. Gomes, N.P. et al. (2006) Genes Dev 20, 601-12.
  4. Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
  5. Buratowski, S. (2009) Mol Cell 36, 541-6.
  6. Wada, T. et al. (1998) EMBO J 17, 7395-403.
  7. Yamada, T. et al. (2006) Mol Cell 21, 227-37.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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