Cell Signaling Technology

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Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb #9030

No. Size Price
9030S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9030 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 92 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb recognizes endogenous levels of β-catenin protein only when acetylated at Lys49.

Acetyl-β-Catenin (Lys49) (D7C2)Rabbit mAb兔单抗可以识别内源性Lys49被乙酰化的β-catenin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing CBP (+), using Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb (upper), β-Catenin (D10A8) XP® Rabbit mAb #8480 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

空载体转染(-)或转染了CBP表达质粒(+)的293T细胞,使用Acetyl-β-Catenin (Lys49) (D7C2)Rabbit mAb(上),β-Catenin (D10A8) XP® Rabbit mAb#8480(中)和β-Actin (D6A8)Rabbit mAb #8457(下)对细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb (upper) or β-Catenin (D10A8) XP® Rabbit mAb #8480 (lower).

使用Acetyl-β-Catenin (Lys49) (D7C2)Rabbit mAb(上)或β-Catenin (D10A8) XP® Rabbit mAb #8480(下)对多种细胞提取物进行western blot分析。


β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游重要的效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤形成(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin 33位和37位丝氨酸以及41位苏氨酸促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin. Research studies have shown that Lys49 is one of few residues frequently mutated in thyroid anaplastic carcinoma (9). CREB-binding protein (CBP) binds and acetylates β-catenin at Lys49 (10,11).


  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Polakis, P. (2000) Genes Dev 14, 1837-51.
  10. Takemaru, K.I. and Moon, R.T. (2000) J Cell Biol 149, 249-54.
  11. Wolf, D. et al. (2002) J Biol Chem 277, 25562-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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