Cell Signaling Technology

Product Pathways - Metabolism

C/EBPα (D56F10) XP® Rabbit mAb (PE Conjugate) #89899

No. Size Price
89899S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
89899 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

C/EBPα (D56F10) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total C/EBPα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human C/EBPα protein.


This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated C/EBPα (D56F10) XP® Rabbit mAb #8178.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and THP-1 cells (green) using C/EBPα (D56F10) XP® Rabbit mAb (PE Conjugate).


CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).

  1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
  2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
  3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
  4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :