Cell Signaling Technology

Product Pathways - Screening Technologies

Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954

motif antibody   phospho-tyrosine   phosphoscan   pY1000   sc-508   sc-7020  

No. Size Price
8954S 100 µl ( 20 western blots ) ¥3,900.00 现货查询 购买询价
8954 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 All Species Expected, Endogenous N/A Rabbit IgG
IP 1:200
F 1:400
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues.

Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb兔单抗能够识别大量酪氨酸磷酸化的蛋白和多肽。该抗体不会与丝氨酸磷酸化或苏氨酸磷酸化的蛋白或多肽产生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.

该单克隆抗体是由含有磷酸化酪氨酸的多肽免疫动物制备。

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, serum-starved (left), treated with H2O2 (2 mM, 10 min; middle), or treated with H2O2 followed by λ phosphatase (right), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

采用共聚焦免疫荧光术分别检测血清饥饿(左图)、 H2O2 (2 mM, 10 min; middle)处理或H2O2后再用λ磷酸酶处理(右图)的C2C12 细胞,使用的抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin标记(红色)。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells, untreated (green) or Gleevec®-treated (blue), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb.

流式细胞术分别检测未处理(绿色)或经Gleevec®处理(蓝色)的K-562细胞活性,使用的抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb. Western blot image was obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology).

Western blot方法分别检测未处理(绿色)或经Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +)处理的A-431细胞提取物,使用的抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb。使用Odyssey® Infrared Imaging System (LI-COR® Biotechnology)进行western blot成像。

IP

IP

Immunoprecipitation of phospho-tyrosine proteins from A-431 cell extracts, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +) (lanes 3 and 4), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb. Western blot analysis was performed using the same antibody. Lanes 1 and 2 are 10% input.

免疫沉淀法分别检测未处理(-)或经Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +)处理的A-431细胞提取物(lanes 3 and 4),使用抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb。Western blot 检测中使用相同抗体。Lane 1为10% input对照。

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

酪氨酸磷酸化在细胞信号转导中扮演重要角色(1)。研究发现,在癌症中,失调的酪氨酸激酶活性会产生不当的增殖和存活信号从而导致恶性肿瘤以及肿瘤形成(2)。针对磷酸化酪氨酸(3,4)的特异性抗体是进行这些研究时非常宝贵的试剂。Cell Signaling Technology研发的磷酸化酪氨酸单克隆抗体具有卓越的灵敏度,可用于研究高通量药物开发中的酪氨酸磷酸化并监测酪氨酸激酶活性。

  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

DRAQ5 is a registered trademark of Biostatus Limited.

LI-COR is a registered trademark of LI-COR, Inc.

Odyssey is a registered trademark of LI-COR, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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