Cell Signaling Technology

Product Pathways - Adhesion

VE-Cadherin (D87F2) XP® Rabbit mAb (PE Conjugate) #89426

No. Size Price
89426S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
89426 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,D. melanogaster,Bovine,Pig, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

VE-Cadherin (D87F2) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total VE-cadherin protein. The antibody does not cross-react with other cadherin family proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VE-cadherin protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated VE-Cadherin (D87F2) XP® Rabbit mAb #2500.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and HUVEC cells (green) using VE-Cadherin (D87F2) XP® Rabbit mAb (PE Conjugate).

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

  1. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
  2. Christofori, G. (2003) EMBO J 22, 2318-23.
  3. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
  4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
  5. Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
  6. Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
  7. Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
  8. Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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