Cell Signaling Technology

Product Pathways - Neuroscience

ApoE4 (4E4) Mouse mAb #8941

No. Size Price
8941S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8941 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 35 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

ApoE4 (4E4) Mouse mAb recognizes endogenous levels of total ApoE4 protein. This antibody does not cross-react with ApoE2 or ApoE3.

ApoE4 (4E4) Mouse mAb 鼠单抗能够检测内源性的ApoE4总蛋白。该抗体不会与ApoE2产生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg130 of human ApoE4 protein.

该单克隆抗体是由合成的人源ApoE4蛋白精氨酸(130位)肽段免疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with a construct expressing full-length human ApoE2 (hApoE2; +), ApoE3 (hApoE3; +), or ApoE4 (hApoE4; +), using ApoE4 (4E4) Mouse mAb (upper) or a pan ApoE antibody (lower).

Western blot方法检测HeLa细胞提取物:全长人ApoE2 (hApoE2; +),ApoE3 (hApoE3; +)或ApoE4 (hApoE4; +)质粒转染;使用的抗体是ApoE4 (4E4) Mouse mAb鼠单抗(上图)或一个广谱型ApoE抗体(下图)。

Western Blotting

Western Blotting

Western blot analysis of human serum using ApoE4 (4E4) Mouse mAb.

Western blot 检测人血清样品,使用的抗体是ApoE4 (4E4) Mouse mAb鼠单抗。

Background

Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2). Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE4 isoform contains arginine at both locations. Research studies have linked ApoE4 function to neuronal plasticity, synaptogenesis, and neurodegenerative diseases (3). ApoE4 is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Investigators have established the ApoE4 allele as a genetic risk factor for Alzheimer’s disease (AD), accounting for 50-60% of the genetic variation in the disease (4). Research studies indicate that patients expressing ApoE4 have a reduced capacity for synaptic plasticity, an earlier age of onset of AD, and an increase in amyloid-beta (Aβ) deposition. The increase in Aβ suggests a role for ApoE4 in the impairment of amyloid clearance (5).

载脂蛋白是血浆脂蛋白,作用于循环系统中的脂质和胆固醇的转运。乳糜微粒是基本的载脂蛋白,含有极低密度脂蛋白(VLDL),中间密度脂蛋白(IDL),低密度脂蛋白(LDL)和高密度脂蛋白(HDL)(1,2)。人类ApoE基因有三种亚型:ApoE2、 ApoE3 和 ApoE4。这三种亚型的不同之处分别位于130位和176位的半胱氨酸和精氨酸残基。 ApoE4亚型在这两个位置都含有精氨酸。研究发现ApoE4作用于神经元的可塑性,突触和神经退行性疾病(3)。 ApoE4主要是在肝脏和大脑产生的,虽然它在其它组织如肺,脾和卵巢等也有广泛表达。研究者已将ApoE4等位基因作为阿尔茨海默氏病(AD)的遗传危险因子,在疾病中遗传变异占50-60%(4)。调查研究表明,表达ApoE4的患者会伴有突触可塑性能力降低,AD发病年龄提早,和β-淀粉样蛋白(Aβ)沉积增加。 Aβ的增加表明了ApoE4对清除淀粉样蛋白的削减功能(5)。

  1. Kwiterovich, P.O. (2000) Am J Cardiol 86, 5L-10L.
  2. Hussain, M.M. (2000) Atherosclerosis 148, 1-15.
  3. Raber, J. et al. Neurobiol Aging 25, 641-50.
  4. Corder, E.H. et al. (1993) Science 261, 921-3.
  5. Holtzman, D.M. et al. (2000) Proc Natl Acad Sci U S A 97, 2892-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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