Cell Signaling Technology

Product Pathways - Transcription Factors

Sox10 (D5V9L) Rabbit mAb #89356

sc-365692  

No. Size Price
89356S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
89356T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
89356 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 62 Rabbit IgG
IP 1:100
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Sox10 (D5V9L) Rabbit mAb recognizes endogenous levels of total Sox10 protein. This antibody also recognizes presumptive sumoylated forms of Sox10 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu249 of human Sox10 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-MEL-5 (positive, left) and HeLa (negative, right) cells using Sox10 (D5V9L) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Sox10 (D5V9L) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells are negative for Sox10 protein.

IP

IP

Immunoprecipitation of Sox10 from A-204 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is Sox10 (D5V9L) Rabbit mAb. Western blot analysis was performed using Sox10 (D5V9L) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

Background

SRY-box 10 (Sox10) is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of cell fate. Sox10 is an important regulator of neural crest and peripheral nervous system development (1-3). Mutations in the SOX10 gene are associated with a group of auditory-pigmentary developmental disorders, including Waardenburg and Waardenburg-Shah syndromes (3,4). Research studies suggest an oncogenic role for Sox10 in various tumor types, such as hepatocellular carcinoma and melanoma (5,6).

  1. Hong, C.S. and Saint-Jeannet, J.P. (2005) Semin Cell Dev Biol 16, 694-703.
  2. Britsch, S. et al. (2001) Genes Dev 15, 66-78.
  3. Bondurand, N. and Sham, M.H. (2013) Dev Biol 382, 330-43.
  4. Mollaaghababa, R. and Pavan, W.J. (2003) Oncogene 22, 3024-34.
  5. Zhou, D. et al. (2014) Tumour Biol 35, 9935-40.
  6. Graf, S.A. et al. (2014) J Invest Dermatol 134, 2212-20.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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