Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Stem Cell Factor (hSCF) #8925

hSCF   KITLG   MGF   scf   Stem cell factor  

No. Size Price
8925LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
8925LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
8925SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
8925SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
8925 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human SCF (hSCF) Glu26-Ala189 (Accession #P21583) was expressed in human 293 cells at Cell Signaling Technology.

CST公司的重组人SCF(hSCF) 蛋白Glu26-Ala189 (Accession #P21583)在人293细胞表达获得。

Molecular Characterization

Recombinant hSCF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,458. DTT-reduced and non-reduced protein migrate as 22-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal EGICR of recombinant hSCF was verified by amino acid sequencing.

重组hSCF蛋白不带标签,非糖基化蛋白分子量是18,458。DTT-还原和非还原蛋白迁移位于22-35 kDa。SDS-PAGE中低迁移速率和蛋白异质性是由糖基化引起的。重组hSCF氨基端EGICR通过蛋白质测序验证。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hSCF. All lots are greater than 98% pure.

重组hSC蛋白F经过还原(+)和非还原(-)SDS-PAGE验证,所有批次纯度大于98%。

Bioactivity

The bioactivity of recombinant hSCF was determined in a M-07e cell proliferation assay. The ED50 of each lot is between 2-6 ng/ml.

重组hSCF的生物活性在M-07e细胞增殖实验中检测,每一批次的ED50在2-6ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hSCF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hSCF and staining overnight with Coomassie Blue.重组hSCF纯度用SDS-PAGE验证,6 µg还原(+)和未还原(-)的重组hSCF电泳,考马斯蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of M-07e cells treated with increasing concentrations of hSCF was assessed. After 48 hour treatment with hSCF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.检测逐渐增长浓度的hSCF对M-07细胞扩增的影响。用hSCF处理48小时候,用一种四唑盐处理细胞,并检测OD450 - OD650吸光值。

Western Blotting

Western Blotting

Western blot analysis of extracts from M-07e cells untreated or treated with hSCF for 5 minutes, using Phospho-c-Kit (Tyr719) Antibody #3391 (upper) and c-Kit Antibody #3392 (lower)用Phospho-c-Kit (Tyr719) 抗体 #3391 (上) 和 c-Kit 抗体#3392 (下)对未处理或经过hSCF处理5分钟的M-07e细胞提取物进行western blot实验。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hSCF.

1ug hSCF含有内毒素量低于0.01ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hSCF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

包含载体蛋白形式:PBS缓冲液,pH 7.2 包含20 μg BSA 每1 μg hSCF用0.22um滤膜过滤后冻干。不含载体蛋白形式:pH 7.2 PBS经过0.22um滤膜过滤后冻干。

Background

SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).

SCF存在于内皮细胞,成纤维细胞,角质化细胞,肠上皮细胞和癌细胞(1,2)。SCF对于造血作用和肥大细胞的分化非常重要,并且也在其他类型细胞的生存等调控中扮演角色(1)。一些肿瘤细胞的增殖和侵袭受SCF蛋白起始(3)。肿瘤来源的SCF似乎涉及骨髓来源的抑制细胞的增殖,这种抑制细胞会限制肿瘤-侵润-T细胞的扩增(4)。SCF在肿瘤微环境中也有其他作用(2)。SCF可以以可溶蛋白状态或嵌膜蛋白状态存在,它的存在形式决定于剪切或蛋白水解释放的不同异构体(1)。SCF可以与酪氨酸激酶受体c-Kit结合,随后激活Akt, Erk, JNK和p38 信号通路 (5,6)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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