Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922

csf2   gm-csf   gmcsf  

No. Size Price
8922SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
8922SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
8922 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human GM-CSF (hGM-CSF) Ala18 - Glu144 (Accession # NM_000758) was produced in E. coli at Cell Signaling Technology.

CST公司生产的人源重组蛋白GM-CSF (hGM-CSF) Ala18 - Glu144 (Accession # NM_000758)是从大肠杆菌细胞表达而来。

Molecular Characterization

Recombinant hGM-CSF does not have a Met on the amino terminus and has a calculated MW of 14477. DTT-reduced protein migrates as a 14 kDa polypeptide and non-reduced protein has slightly greater mobility due to intramolecular cystines. The expected amino-terminal APARS of recombinant hGM-CSF was verified by amino acid sequencing.

重组的hGM-CSF蛋白在N-末端没有Met标签,蛋白分子量据推算为14,477Da。DTT-降解蛋白作为一个14kDa的蛋白转移。未降解的蛋白的迁移率稍高是因为分子内的胱氨酸存在。重组hGM-CSF 蛋白的N-末端APARS的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hGM-CSF. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hIL-17A/F通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant hGM-CSF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 5-30 pg/ml.

hGM-CSF的生物活性是通过TF-1细胞增殖来确定的。每个批次的ED50在5-30 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hGM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hGM-CSF and staining overnight with Coomassie Blue.重组hGM-CSF蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hGM-CSF 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from TF-1 cells, untreated or treated with hGM-CSF for 10 minutes, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (upper) and Stat5 (3H7) Rabbit mAb #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (上图)和Stat5 (3H7) Rabbit mAb #9358 (下图) 研究未经处理的和经hGM-CSF 处理10 min的TF-1 细胞的细胞提取液。



The proliferation of TF-1 cells treated with increasing concentrations of hGM-CSF was assessed. After 48 hour treatment with hGM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在hGM-CSF 蛋白浓度递增条件下研究TF-1细胞增殖实验。用hGM-CSF 培养细胞48小时后用四唑盐处理。并测定OD450 - OD650值。


Less than 0.01 ng endotoxin/1μg hGM-CSF.

内毒素含量:小于0.01 ng /1 μg hGM-CSF。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hGM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hGM-CSF蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hGM-CSF蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


GM-CSF is produced by activated T cells, NK cells and macrophages (1,5). Target cells include granulocyte, monocyte precursors and subsets of differentiated myeloid cells (1,2,3). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in hematopoietic differentiation of dendritic cells and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (4). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5 and PI3K/Akt pathways (1).

GM-CSF 是通过活化的T细胞,NK细胞和巨噬细胞产生(1,5)。靶标细胞包括粒性白细胞,单核细胞前体和经过分化的一系列的骨髓细胞(1,2,3)。很多的靶标细胞需要GM-CSF才能生存。GM-CSF 诱导增殖,并参与造血细胞的分化成树状细胞,它在干细胞分化的信号通路上也是主要的因素之一。GM-CSF 激活骨髓细胞的功能,所以它连先天免疫和获得性免疫并推进抗肿瘤免疫(4)。GM-CSF受体由GM-CSFRα和普遍的 β链, βC 组成,这同时也被IL-3和 IL-5所利用(1)。GM-CSF的结合起始了 Jak2, Stat5 和 PI3K/Akt 信号通路(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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