Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Interleukin-4 (hIL-4) #8919

hIL-4   IL-4   IL4   Interleukin-4  

No. Size Price
8919SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
8919SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
8919 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human IL-4 (hIL-4) His25-Ser153 (Accession #AF395008) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的人源重组蛋白IL-4 (hIL-4) His25-Ser153 (Accession #AF395008)是从人源293细胞表达而来。

Molecular Characterization

Recombinant hIL-4 does not have a Met on the amino terminus and the nonglycosylated protein has a calculated MW of 14,963. DTT-reduced and non-reduced protein migrate as larger 20 kDa polypeptides due to glycosylation, with non-reduced having slightly greater mobility due to an intramolecular cystine. The expected amino-terminal HKCDI of recombinant hIL-4 was verified by amino acid sequencing.重组的hIL-4蛋白在N-末端没有Met标签,未糖基化的蛋白分子量据推算为14,963。DTT-降解蛋白和未降解蛋白由于糖基化的存在而作为一个26kDa的蛋白转移。未降解的蛋白的迁移率稍高是因为分子内的胱氨酸存在。重组hIL-4蛋白的N-末端HKCDI的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-4. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hIL-4通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant hIL-4 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 80-250 pg/ml.


Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-4 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-4 and staining overnight with Coomassie Blue.



The proliferation of TF-1 cells treated with increasing concentrations of hIL-4 was assessed. After 48 hour treatment with hIL-4, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

Western Blotting

Western Blotting

Western blot analysis of extracts from TF-1 cells, untreated or treated with hIL-4 for 20 minutes, using Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb Antibody #9364 (upper) and Stat6 Antibody #9362 (lower).


Less than 0.01 ng endotoxin/1 μg hIL-4.

内毒素含量:小于0.01 ng /1 μg hIL-4。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-4. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hIL-4蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hIL-4蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


IL-4 is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces differentiation of naive T cells into the TH2 phenotype. IL-4 also promotes B cell proliferation, antibody isotype switching and expression of other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (2). IL-4 binds to two distinct receptors, the type I receptor and type II receptor. Type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (3,4). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (3). The type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (3).

IL-4通过T 细胞, NK T 细胞, γδ 细胞和肌肉细胞产生(1)。目标细胞包括B 细胞, T 细胞和巨噬细胞(1)。IL-4 诱导T细胞分化为TH2表型。IL-4也能促进B细胞增殖、抗体类型转换、以及其它TH2细胞分泌因子—IL-5和IL-9细胞因子的表达。IL-4诱导TH2的极化,对体液免疫病原菌的发展有重要的作用(1),同时也参与到敏感症和哮喘的发病过程中(2)。IL-4 结合到两个不同的受体上, type I 受体和type II 受体。Type I 受体是异源二聚体包含IL-4Rα 链和普遍存在的 gamma chain, γc (3,4)。Type II 受体, 与IL-13受体共享, 是IL-4Rα 和 IL-13Rα1的二聚体。通过type I受体进行传递的信号能激活 Jak1/Stat6, Jak3 和 PI3K/Akt通路 (3)。type II 受体能激活 Jak1/Stat6和Tyk2/Stat3通路(3)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :