Product Pathways - Growth Factors/Cytokines
Human Epidermal Growth Factor (hEGF) #8916
|8916LC||250 µg ( With Carrier )||￥6,860.00 现货查询||购买询价|
|8916LF||250 µg ( Carrier Free )||￥6,860.00 现货查询||购买询价|
|8916SF||50 µg ( Carrier Free )||￥2,321.00 现货查询||购买询价|
|8916||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant human EGF (hEGF) Asn971-Arg1023 (Accession #NM_0011963) was produced in E. coli at Cell Signaling Technology.
在Cell Signaling Technology公司的重组人源EGF (hEGF)天冬氨酸971-精氨酸1023（编号NM_0011963）经在大肠杆菌中表达生产。
Recombinant hEGF has a Met on the amino terminus and has a calculated MW of 6353. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSDS of recombinant hEGF was verified by amino acid sequencing.
重组人源EGF在N-氨基末端有一个甲基化，分子量为6353. DTT还原和非还原蛋白游离基团为6kDa多肽。 预期在重组人源EGF的N-氨基末端MNSDS由氨基酸测序验证。
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEGF. All lots are greater than 98% pure.
6 μg还原和非还原的重组人源EGF 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。
The bioactivity of recombinant hEGF was determined in a MCF10A cell proliferation assay. The ED50 of each lot is between 0.10-0.60 ng/ml.
重组人源EGF的生物活性由MCF10A细胞增殖实验测定。其每个批次的再ED50在 0.10-0.60 ng/ml之间。
The purity of recombinant hEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hEGF and staining overnight with Coomassie Blue.重组hFGF basic 的纯度用SDS-PAGE验证，6 µg 还原（-）和未还原（-）重组hFGF basic 电泳后考马斯蓝染色过夜。
Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF for 10 minutes, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper) and EGF Receptor Antibody #2232 (lower).用Phospho-EGF Receptor (Tyr1173) (53A5)兔单抗#4407(上) 和EGF Receptor 抗体#2232(下) 对未处理或经过hEGF处理10分钟的A-431细胞提取物进行western blot实验。
The proliferation of MCF10A cells treated with increasing concentrations of hEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验浓度逐渐增长的hFGF basic 对MCF10A细胞增殖能力的影响。细胞在孵育24小时后，用BrdU处理4小时。BrdU掺入使用ELISA验证并检验OD450-OD690 。
Less than 0.01 ng endotoxin/1 μg hEGF.
每1 μg hEGF中所含内毒素小于0.01 ng。
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
有载体：每1 μg hEGF由含有20 μg BSA的pH 7.2的PBS 0.22微米过滤冻干。无载体：pH 7.2的PBS 0.22微米过滤冻干。
EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1). Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).
- Kovacevic, Z. et al. (2016) J Biol Chem 291, 1029-52.Applications:
- Cursons, J. et al. (2015) Cell Commun Signal 13, 26.Applications:
- Schröder, S. et al. (2013) Mol Cell 52, 314-24.Applications:
- Herr, D.R. et al. (2013) J Biol Chem 288, 29882-9.Applications:
- Williams, K.A. et al. (2013) J Biol Chem 288, 33156-70.Applications:
- Tsai, C.Y. et al. (2012) Biochem Pharmacol 84, 1007-13.Applications:
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 2231 Phospho-EGF Receptor (Tyr845) Antibody
- 2232 EGF Receptor Antibody
- 2234 Phospho-EGF Receptor (Tyr1068) Antibody
- 2235 Phospho-EGF Receptor (Tyr992) Antibody
- 2236 Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
- 2237 Phospho-EGF Receptor (Tyr1045) Antibody
- 2238 Phospho-EGF Receptor (Ser1046/1047) Antibody
- 2239 EGF Receptor (1F4) Mouse mAb
- 2256 EGF Receptor (EGFR1) Mouse mAb (IP Specific)
- 2646 EGF Receptor (C74B9) Rabbit mAb
- 2963 EGF Receptor (T43) Antibody
- 3265 EGF Receptor (V279) Antibody
- 4405 EGF Receptor (15F8) Rabbit mAb
- 4407 Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
- 8102 SignalSlide® Phospho-EGF Receptor IHC Controls
- 9872 Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)
- 9922 Phospho-EGF Receptor Antibody Sampler Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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