Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Epidermal Growth Factor (hEGF) #8916

EGF   EGF Receptor   EGFR   Epidermal Growth Factor   hEGF   Human EGF  

No. Size Price
8916LC 250 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
8916LF 250 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
8916SC 50 µg ( With Carrier ) 请询价 现货查询 购买询价
8916SF 50 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
8916 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human EGF (hEGF) Asn971-Arg1023 (Accession #NM_0011963) was produced in E. coli at Cell Signaling Technology.

在Cell Signaling Technology公司的重组人源EGF (hEGF)天冬氨酸971-精氨酸1023(编号NM_0011963)经在大肠杆菌中表达生产。

Molecular Characterization

Recombinant hEGF has a Met on the amino terminus and has a calculated MW of 6353. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSDS of recombinant hEGF was verified by amino acid sequencing.

重组人源EGF在N-氨基末端有一个甲基化,分子量为6353. DTT还原和非还原蛋白游离基团为6kDa多肽。 预期在重组人源EGF的N-氨基末端MNSDS由氨基酸测序验证。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEGF. All lots are greater than 98% pure.

6 μg还原和非还原的重组人源EGF 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。

Bioactivity

The bioactivity of recombinant hEGF was determined in a MCF10A cell proliferation assay. The ED50 of each lot is between 0.10-0.60 ng/ml.

重组人源EGF的生物活性由MCF10A细胞增殖实验测定。其每个批次的再ED50在 0.10-0.60 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hEGF and staining overnight with Coomassie Blue.重组hFGF basic 的纯度用SDS-PAGE验证,6 µg 还原(-)和未还原(-)重组hFGF basic 电泳后考马斯蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF for 10 minutes, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper) and EGF Receptor Antibody #2232 (lower).用Phospho-EGF Receptor (Tyr1173) (53A5)兔单抗#4407(上) 和EGF Receptor 抗体#2232(下) 对未处理或经过hEGF处理10分钟的A-431细胞提取物进行western blot实验。

Bioactivity

Bioactivity

The proliferation of MCF10A cells treated with increasing concentrations of hEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验浓度逐渐增长的hFGF basic 对MCF10A细胞增殖能力的影响。细胞在孵育24小时后,用BrdU处理4小时。BrdU掺入使用ELISA验证并检验OD450-OD690 。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hEGF.

每1 μg hEGF中所含内毒素小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每1 μg hEGF由含有20 μg BSA的pH 7.2的PBS 0.22微米过滤冻干。无载体:pH 7.2的PBS 0.22微米过滤冻干。

Background

EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

EGF存在于上皮细胞,成纤维细胞和许多其他的细胞类型中(1,2)。低分子量可溶型EGF由一个分子量超过130,000的跨膜前体蛋白经蛋白水解生成(1,2)。可溶型和跨膜EGF都是有活性的(2)。EGF可以诱导多种细胞包括肿瘤来源的细胞增殖,分化和存活(1-3)。EGF家族和ErbB/HER EGF受体家族有许多成员。EGF结合ErbB1/HER1诱导同源二聚化或与其他ErbB/HER成员的异源二聚化(1)。EGF,EGF家族成员,EGF受体和它们的信号通路涉及到多种癌症,可以作为介入治疗的靶点(1,2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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