Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913

PDGF   pdgf-aa   PDGFA   pdgfaa  

No. Size Price
8913LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
8913LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
8913SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
8913SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
8913 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human PDGF-AA (hPDGF-AA) Ser87-Thr211 (Accession #NP_002598) was produced in E. coli at Cell Signaling Technology.

CST公司的人源重组蛋白PDGF-AA (hPDGF-AA) Ser87-Thr211 (Accession #NP_002598)经过大肠杆菌表达纯化获得。

Molecular Characterization

Recombinant hPDGF-AA does not have a Met on the amino terminus and has a calculated MW of 14,305. DTT-reduced protein migrates as an 18 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 34 kDa protein. The expected amino-terminal SIEEA of recombinant hPDGF-AA was verified by amino acid sequencing.

重组的hPDGF-AA蛋白在末端没有Met标签,其分子量据推算为14,305。DTT-降解蛋白作为一个18kDa的蛋白而转移,未降解的蛋白由胱氨酸形成二聚体作为一个34kDa的蛋白而转移。重组hPDGF-AA蛋白的N-末端SIEEA的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-AA. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hPDGF-AA was determined in a NIH/3T3 proliferation assay. The ED50 of each lot is between 4-12 ng/ml.

重组蛋白hPDGF-AA的生物活性是通过NIH/3T3细胞的增殖实验确定的。每个lot的ED50值在4-12 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hPDGF-AA was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hPDGF-AA and staining overnight with Coomassie Blue.重组hPDGF-AA 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hPDGF-AA 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of NIH/3T3 cells treated with increasing concentrations of hPDGF-AA was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD650 was determined.在hPDGF-AA 蛋白浓度递增条件下研究NIH/3T3 细胞增殖实验。用hPDGF-AA 培养细胞24小时后用BrdU标记4小时。BrdU的结合通过ELISA和测定OD450 - OD650值。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with hPDGF-AA for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 和 Akt1 (C73H10) Rabbit mAb #2938 (下图) 研究未经处理的和经 hPDGF-AA 处理10 min的NIH/3T3 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hPDGF-AA.

每1 μg hPDGF-AA内毒素含量少于0.01 ng 。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hPDGF-AA. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

有载体: 蛋白溶解在20 mM 柠檬酸盐, pH 3.0 包含 100 mM NaCl 和 20 μg BSA/1 μg hPDGF-AA的溶液中,并通过 0.22 μm 滤膜冻干。无载体:蛋白溶解在20 mM 柠檬酸盐, pH 3.0 包含 100 mM NaCl,并通过 0.22 μm 滤膜冻干。

Background

PDGF-AA is integrally involved in embryonic development, angiogenesis and organogenesis and induces fibroblast proliferation and migration (1,2). PDGF-AA is produced by epithelial, muscle, osteosarcoma and neuronal progenitor cells (1,3). Active PDGF-AA is formed through intracellular proteolytic cleavage of a large precursor. PDGF-AA is also concentrated in the extracellular matrix through alternative splicing that generates an extended carboxy-terminal that binds components of the extracellular matrix. The carboxy-terminal stretch is removed extracellularly to generate mature PDGF-AA (1,2). PDGF-AA binding to the PDGFR-α activates the receptor tyrosine kinase (1). PDGF-AA-induced signaling is through the Ras-MAPK, PI3K/AKT and PLCγ pathways (1). Dysregulation of PDGF-AA expression and/or signaling is often associated with cancer and fibrotic disorders (1).

PDGF-AA参与到胚胎的发育,血管生成和器官发育和诱导纤维原细胞的增殖和迁移等过程(1,2)。PDGF-AA是通过上皮,肌肉,骨肉瘤神经元细胞而产生(1,3)。活性的PDGF-AA是通过胞内蛋白酶剪切一个很大的前体而形成。PDGF-AA 通过可变剪切产生一个伸长的C-末端集合细胞基质中的成分并得到富集。C-末端的伸长在细胞外被剪切而生成成熟的 PDGF-AA (1,2)。PDGF-AA 与PDGFR-α结合结合激活酪氨酸激酶受体(1)。PDGF-AA诱导的信号传导经过Ras-MAPK, PI3K/AKT 和 PLCγ 通路 (1)。PDGF-AA表达或信号通路的失调通常与癌症和纤维化失调有关系(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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