Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912

hPDGF-b   pdgf-b   pdgf-bb   PDGFb  

No. Size Price
8912SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
8912SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
8912 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human PDGF-BB (hPDGF-BB) Ser67-Thr190 (Accession #NP_002599) was produced in E. coli at Cell Signaling Technology.

Cell Signaling Technology的重组人源PDGF-BB(hPDGF-BB)丝氨酸67-苏氨酸190(编号NP_002599)经在E.coli中表达产生。

Molecular Characterization

Recombinant hPDGF-BB does not have a Met on the amino terminus and has a calculated MW of 12,294. DTT-reduced protein migrates as a 14 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 32 kDa protein. The expected amino-terminal SLGSL of recombinant hPDGF-BB was verified by amino acid sequencing.



>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-BB. All lots are greater than 98% pure.

6 μg还原和非还原的重组hPDGF-BB 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。


The bioactivity of recombinant hPDGF-BB was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 4.0 - 15 ng/ml.

重组的hPDGF-BB的生物活性由NIH/3T3细胞增殖实验测定。其每个批次的ED50值在 4.0-15 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hPDGF-BB was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hPDGF-BB and staining overnight with Coomassie Blue.重组hPDGF-BB 的纯度通过SDS-PAGE验证,6 µg 还原(-)和未还原(-)重组hPDGF-BB 电泳后考马斯蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with hPDGF-BB for 10 minutes, using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).使用Phospho-Akt (Thr308) (C31E5E) 兔单抗 #2965 (上) 和Akt1 (C73H10) 兔单抗#2938 (下)对未处理或经过hPDGF-BB处理10分钟的NIH/3T3细胞提取物进行western blot实验。



The proliferation of NIH/3T3 cells treated with increasing concentrations of hPDGF-BB basic was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验浓度逐渐增长的hPDGF-BB basic 对 NIH/3T3细胞增殖的影响。细胞在孵育24小时后,用BrdU处理4小时。BrdU掺入使用ELISA验证并检验OD450-OD690 。


Less than 0.01 ng endotoxin/1 μg hPDGF-BB.

每1 μg hPDGF-BB产品所含内毒素少于0.01ng。


With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hPDGF-BB. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

有载体的:每1 μg hPDGF-BB溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干。无载体的:经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干。


PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRβ and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways (1, 2). PDGF-BB may have a role in some cancer types (2).PDGF-BB may have a role in some cancer types (2).

PDGF-BB是由胱氨酸连接的二聚体。PDGF家族成员成员在发育、细胞增殖、细胞存活和血管生成中起着关键作用(1,2)。PDGF-BB在血管内皮、巨核细胞和睾丸间质细胞中表达[2]。PDGF-BB靶标外皮细胞、成纤维细胞、内皮细胞和其他类型细胞[1-3]。PDGF 诱导成纤维细胞的生长和迁移[3]。对于单核细胞和粒细胞来说,PDGF是一种化学引诱物。前体的PDGF-BB在胞内被剪切而产生一个含有伸展的C末端的形式,以帮助保持PDGF-BB在细胞外基质。在第二次剪接中,伸展的C末端在胞外被除去而形成PDGF-BB成熟体[1,2]。PDGF-BB 与 PDGFRβ结合诱导受体的二聚化作用并通过PI3K/Akt, JNK,和 PLCγ通路传递信号[1,2]。PDGF-BB可能在某些癌症中起作用[2]。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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