Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912

hPDGF-b   pdgf-b   pdgf-bb   PDGFb  

No. Size Price
8912LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
8912LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
8912SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
8912SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
8912 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human PDGF-BB (hPDGF-BB) Ser67-Thr190 (Accession #NP_002599) was produced in E. coli at Cell Signaling Technology.

Cell Signaling Technology的重组人源PDGF-BB(hPDGF-BB)丝氨酸67-苏氨酸190(编号NP_002599)经在E.coli中表达产生。

Molecular Characterization

Recombinant hPDGF-BB does not have a Met on the amino terminus and has a calculated MW of 12,294. DTT-reduced protein migrates as a 14 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 32 kDa protein. The expected amino-terminal SLGSL of recombinant hPDGF-BB was verified by amino acid sequencing.

重组的hPDGF-BB再N-氨基末端不含甲基化,经计算分子量为12,294。DTT-还原的蛋白游离体为14kDa多肽,胱氨酸连接的非还原二聚体游离物为32kDa蛋白。重组的hPDGF-BB的预期的N-氨基末端SLGSL由氨基酸测序验证。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-BB. All lots are greater than 98% pure.

6 μg还原和非还原的重组hPDGF-BB 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。

Bioactivity

The bioactivity of recombinant hPDGF-BB was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 4.0 - 15 ng/ml.

重组的hPDGF-BB的生物活性由NIH/3T3细胞增殖实验测定。其每个批次的ED50值在 4.0-15 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hPDGF-BB was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hPDGF-BB and staining overnight with Coomassie Blue.重组hPDGF-BB 的纯度通过SDS-PAGE验证,6 µg 还原(-)和未还原(-)重组hPDGF-BB 电泳后考马斯蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with hPDGF-BB for 10 minutes, using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).使用Phospho-Akt (Thr308) (C31E5E) 兔单抗 #2965 (上) 和Akt1 (C73H10) 兔单抗#2938 (下)对未处理或经过hPDGF-BB处理10分钟的NIH/3T3细胞提取物进行western blot实验。

Bioactivity

Bioactivity

The proliferation of NIH/3T3 cells treated with increasing concentrations of hPDGF-BB basic was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验浓度逐渐增长的hPDGF-BB basic 对 NIH/3T3细胞增殖的影响。细胞在孵育24小时后,用BrdU处理4小时。BrdU掺入使用ELISA验证并检验OD450-OD690 。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hPDGF-BB.

每1 μg hPDGF-BB产品所含内毒素少于0.01ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hPDGF-BB. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

有载体的:每1 μg hPDGF-BB溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干。无载体的:经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干。

Background

PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRβ and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways (1, 2). PDGF-BB may have a role in some cancer types (2).PDGF-BB may have a role in some cancer types (2).

PDGF-BB是由胱氨酸连接的二聚体。PDGF家族成员成员在发育、细胞增殖、细胞存活和血管生成中起着关键作用(1,2)。PDGF-BB在血管内皮、巨核细胞和睾丸间质细胞中表达[2]。PDGF-BB靶标外皮细胞、成纤维细胞、内皮细胞和其他类型细胞[1-3]。PDGF 诱导成纤维细胞的生长和迁移[3]。对于单核细胞和粒细胞来说,PDGF是一种化学引诱物。前体的PDGF-BB在胞内被剪切而产生一个含有伸展的C末端的形式,以帮助保持PDGF-BB在细胞外基质。在第二次剪接中,伸展的C末端在胞外被除去而形成PDGF-BB成熟体[1,2]。PDGF-BB 与 PDGFRβ结合诱导受体的二聚化作用并通过PI3K/Akt, JNK,和 PLCγ通路传递信号[1,2]。PDGF-BB可能在某些癌症中起作用[2]。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications