Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Vascular Endothelial Growth Factor-121 (hVEGF121 ) #8908

No. Size Price
8908SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
8908SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
8908 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human VEGF121 (hVEGF121) Ala207-Arg327 (Accession #NP_001020541.2) was expressed in human 293 cells at Cell Signaling Technology.

CST公司在人293细胞中生产重组人蛋白VEGF121 (hVEGF121) Ala207-Arg327 (Accession #NP_001020541.2)。

Molecular Characterization

Recombinant hVEGF121 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,057. DTT-reduced protein migrates as a 14-22 kDa polypeptide. Heterogeneity in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 30-36 kDa protein. The expected amino-terminal APMAE of recombinant hVEGF121 was verified by amino acid sequencing.

重组的hVEGF121蛋白没有标签,未糖基化蛋白的分子量据推算为14,057Da。DTT-还原蛋白迁移时是作为14-22 kDa 的多肽而转移。在SDS-PAGE中不同性质是由于糖基化造成。未还原的蛋白通过胱氨酸形成二聚体并作为30-36 kDa 多肽而迁移。重组hVEGF121蛋白的N-末端APMAE的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hVEGF121. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hVEGF121通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant hVEGF121 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 0.5-2 ng/ml.

重组蛋白hVEGF121的生物活性是通过HUVEC细胞的细胞增殖实验确定的。每个批次的ED50在0.5-2 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hVEGF121 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hVEGF121 and staining overnight with Coomassie Blue.重组蛋白hVEGF121的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。



The proliferation of HUVEC treated with increasing concentrations of hVEGF121 was assessed. After 72-hour treatment with hVEGF121 cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined. 用递增浓度的hVEGF121处理HUVEC 并验证增殖的实验。用 hVEGF121 处理72小时后,细胞与四唑盐孵育,然后测定OD450 - OD650数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC untreated or treated with hVEGF121 for 15 minutes, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) and p38 MAPK Antibody #9212 (lower).Western免疫印迹。用Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215(上图)和p38 MAPK Antibody #9212(下图)研究未经处理和经hVEGF121 处理15分钟的 HUVEC细胞的细胞提取液。


Less than 0.01 ng endotoxin/1μg hVEGF121.

内毒素含量:<0.01 ng /1 μg hVEGF121。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hVEGF121. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hVEGF121蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hVEGF121蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


VEGF121 is the second most abundant splice variant of VEGF-A (1,2). VEGF121 is produced by endothelial cells, macrophages, T-cells and other cell types. VEGF121 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1). VEGF121 expression is induced by hypoxia, inflammatory cytokines and through oncogene products in tumors (1-3). VEGF121 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF121 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/AKT, P38 MAPK, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

VEGF121是VEGF-A不同剪切中数量居第二的形式(1,2)。VEGF121是通过内皮细胞、巨噬细胞、T细胞和其它的一些细胞类型产生。VEGF121参与到了血管再生、血管内皮细胞生存、生长、迁移和血管渗透(1)。VEGF121的表达受到组织缺氧,炎症因子和肿瘤组织产生的致癌基因的诱导(1-3)。VEGF121结合到VEGFR1和VEGFR2 受体酪氨酸激酶(1)。VEGF121 结合到 VEGFR1和VEGFR2导致了信号通路的激活,如PI3K/AKT, P38 MAPK和 FAK (1)。在很多癌症中,VEGF在肿瘤血管再生中发挥了重要的作用(2)。

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