Product Pathways - Growth Factors/Cytokines
Human Interleukin-1β (hIL-1β) #8900
|8900LC||50 µg ( With Carrier )||￥10,050.00 现货查询||购买询价|
|8900LF||50 µg ( Carrier Free )||￥10,050.00 现货查询||购买询价|
|8900SC||10 µg ( With Carrier )||￥2,580.00 现货查询||购买询价|
|8900SF||10 µg ( Carrier Free )||￥2,580.00 现货查询||购买询价|
|8900||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant human IL-1β (hIL-1β) Ala117-Ser269 (Accession #NM_000576) was produced in E. coli at Cell Signaling Technology.
CST公司在大肠杆菌中生产重组人蛋白IL-1β (hIL-1β) Ala117-Ser269 (Accession #NM_000576)。
Recombinant hIL-1β does not have a Met on the amino terminus and has a calculated MW of 17,377. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal APVRS of recombinant hIL-1β was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1β. All lots are greater than 98% pure.
6 μg 还原 (+) 和 非还原的(-) hIL-1β通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。
The bioactivity of recombinant IL-1β was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-20 pg/ml.
The purity of recombinant hIL-1β was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-1β and staining overnight with Coomassie Blue.重组蛋白hIL-1β的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。
The production of IL-8 by primary human fibroblasts cultured with increasing concentrations of human IL-1β was assessed. Media from cells incubated with IL-1β for 24 hours was collected and assayed for IL-8 by ELISA and the OD450-OD650 was determined.用递增浓度的hIL-1β孵育诱导人成纤维原代细胞产生IL-8实验。用hIL-1β培养细胞24小时并收集上清，人IL-8用ELISA 实验测定，并测定OD450-OD650 数值。
Western blot analysis of extracts from MCF-7 cells, untreated or treated with hIL-1β for 20 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 Antibody #3034 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图)和NF-κB p65 Antibody #3034 (下图)研究未经处理和经hIL-1β处理20分钟的MCF7细胞的细胞提取液。
Less than 0.01 ng endotoxin/1 μg hIL-1β.
内毒素含量:<0.01 ng /1 μg hIL-1β。
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
有载体: 每微克hIL-1β蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。
无载体：每微克hIL-1β蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。
IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).
IL-1β是促炎症因子，主要的由激活的单核细胞和上皮细胞产生(1)。IL-1β的前体被caspase-1剪切，成熟的IL-1β紧接着被分泌(1-3)。目标细胞包括巨噬细胞和许多其它的细胞类型。IL-1β引起的信号通路包括IL-1β结合到IL-1的附属蛋白 (IL-1-AcP); 此复合体紧接着结合到IL-1RI (1,2)。信号通过激活MAP激酶和 NF-κB信号通路(1,2)。IL-1β也结合到IL-1RII,它缺乏细胞内的信号区域并因此作为高亲和性的诱捕受体。IL-1β结合到IL-1RI的过程能够被负调控因子IL-1R拮抗剂IL-1Ra所抑制。IL-1Ra结合到IL-1RI后无信号传导并且作为抑制剂阻止IL-1β信号通路。IL-1β在急性应答期和脓毒病中发挥了重要的作用(1-3)。
- Cheng, H.H. et al. (2014) PLoS One 9, e88507.Applications:
- Fischer, A. et al. (2014) Am J Physiol Gastrointest Liver Physiol 306, G218-28.Applications:
- Kotobuki, Y. et al. (2012) Pigment Cell Melanoma Res 25, 219-30.Applications:Growth factors/cytokines,
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- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 9872 Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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