Cell Signaling Technology

Product Pathways - Companion Products

Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8887

anti mouse   PE   R-PE   secondary  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
F Goat

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.



Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Anti-mouse IgG (H+L), F(ab')2 Fragment在最适条件下与藻红蛋白(PE)结合,产品规格为1 mg/ml。本F(ab')2 fragment通过Fc受体非特异性结合到细胞的情况较少。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).流式细胞检测分析未处理的Jurkat细胞,使用Akt (5G3) Mouse mAb #2966检测Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate)(蓝色),同时用Mouse (G3A1) mAb IgG1 Isotype Control #5415(红色)作为同型对照。


This product has been optimized for use as a secondary antibody in FLOW ctytometry.


Directions for Use

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.



Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.


Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :