Cell Signaling Technology

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Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885

anti-rabbit   PE   phycoerythrin   secondary  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
F Goat

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.

F(ab')2片段由山羊抗体制备,经混合的人和小鼠血清、浆细胞瘤/杂交瘤蛋白和纯化的人副蛋白类吸附。

Description

Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Anti-rabbit IgG (H+L), F(ab')2 Fragment在最适条件下与藻红蛋白(PE)结合,产品规格为1 mg/ml。本F(ab')2 fragment通过Fc受体非特异性结合到细胞的情况较少。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate).流式细胞检测分析Jurkat细胞,未处理(绿色)或用LY294002 #9901、Wortmannin #9951和U0126 #9903(蓝色)处理,使用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060来检测Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate)。

Applications

This product has been optimized for use as a secondary antibody in FLOW ctytometry.

本产品是经过优化的流式细胞术的二抗。

Directions for Use

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.

二抗的最佳稀释度根据实际应用来确定。最终稀释度为1:500时,对于大多数的流式细胞分析都能得出可取的结果。

Background

Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

荧光标记的抗物种IgG结合物是用于流式细胞术和免疫荧光的理想产品。CST公司严格的质量控制规程确保每个结合物都能提供最理想的特异性和荧光。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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