Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb #8842

No. Size Price
8842S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
8842T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
8842 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 140 Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb recognizes endogenous levels of TACC3 protein only when phosphorylated at Ser558. Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb能够识别内源性Ser558磷酸化的TACC3蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser558 of human TACC3 protein. 该单克隆抗体是由合成的针对人TACC3蛋白的558位丝氨酸的肽段免疫动物生产的。



Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测HT-29 细胞,左图未处理,右图为λ磷酸酶处理的,使用的抗体为Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb (绿色)。蓝色伪彩为DNA荧光染料(产品信息为 DRAQ5®#4084 )。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated (-) or synchronized in mitosis by thymidine block (2 mM, 17 hr) followed by nocodozole treatment (100 ng/ml, 24 hr) (+), using Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb. Western blot方法检测HT-29细胞,未处理(-)或者是伴随诺考达唑处理 (100 ng/ml, 24 hr) (+)的胸腺嘧啶阻遏(2 mM, 17 hr)引起有丝分裂同步化,使用的抗体为Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb。


Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end (1). Three family members have been identified in humans: TACC1, TACC2, and TACC3. These proteins are thought to be involved in centrosomal microtubule assembly and have been mapped to chromosomal regions that are disrupted in some cancers (reviewed in 2). TACC3 has been shown to be upregulated in many cancer cell lines (3). When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability (4,5). For this reason, it has been suggested that monitoring the localization of phosphorylated TACC3 would be an effective way to determine the efficacy of Aurora A inhibitors that show promise as anti-cancer drugs (6,7). In addition, studies have shown that TACC3 could be useful as a prognostic marker for non-small cell lung cancer (8). 转化酸性卷曲螺旋蛋白(TACC)是一个蛋白家族,其特征是羧基末端共有的约200个氨基酸的卷曲螺旋模体(1)。人类中已经鉴定出三个家族成员:TACC1,TACC2和TACC3。这些蛋白被认为参与中心体微管装配且其染色体区域定位在一些癌症中是被破坏的(2中已论述)。TACC3被认为在多种癌细胞系中表达上调(3)。当其558位丝氨酸被极光激酶A磷酸化时,哺乳动物TACC3向有丝分裂纺锤体定位且增加微管的稳定性(4,5)。鉴于此,监控磷酸化TACC3的定位将是确定极光激酶A抑制剂效力的有效方式,而极光激酶A抑制剂是很有前景的抗癌药物(6,7)。此外,研究发现TACC3是一个有效的非小细胞肺癌的预后标志物(8)。

  1. Gergely, F. et al. (2000) Proc Natl Acad Sci USA 97, 14352-7.
  2. Peset, I. and Vernos, I. (2008) Trends Cell Biol 18, 379-88.
  3. Still, I.H. et al. (1999) Genomics 58, 165-70.
  4. Kinoshita, K. et al. (2005) J Cell Biol 170, 1047-55.
  5. Schneider, L. et al. (2007) J Biol Chem 282, 29273-83.
  6. LeRoy, P.J. et al. (2007) Cancer Res 67, 5362-70.
  7. Tyler, R.K. et al. (2007) Cell Cycle 6, 2846-54.
  8. Jung, C.K. et al. (2006) Pathol Int 56, 503-9.

Application References

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