Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMARCC2/BAF170 Antibody #8829

No. Size Price
8829S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8829 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 162, 170 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig, Horse,

Specificity / Sensitivity

SMARCC2/BAF170 Antibody recognizes endogenous levels of total SMARCC2/BAF170 protein (both isoforms 1 and 2).

SMARCC2/BAF170 Antibody能够检测内源性SMARCC1/BAF155总蛋白(亚型1和2)。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human SMARCC2/BAF170 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SMARCC2/BAF170 Antibody.

使用SMARCC2/BAF170 Antibody,免疫印迹(Western blot)分析不同细胞中SMARCC2/BAF170蛋白水平。


ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors such as nuclear receptors, p53, Rb, BRCA1, and MyoD facilitate recruitment of the complex to target genes to regulate gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

SMARCC2/BAF170 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by Brg1 in vitro (10). While SMARCC2/BAF170 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2/BAF170 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2/BAF170 leads to loss of stem cell pluripotency and self renewal (11).


SMARCC2/BAF170是SWI/SNF复合物的中心亚单位之一,在体内该蛋白通过Brg1进行高效的核小体修饰是必须的(10)。在非多能干细胞中SMARCC2/BAF170已经显示是SWI/SNF复合物的部分,而在多能胚胎肝 (ES)细胞中是缺少的。SMARCC2/BAF170蛋白表达已经显示在神经元/神经祖细胞中是上调的,这些细胞是来自含视黄酸的小鼠ES细胞分化的,并且SMARCC2/BAF170蛋白外源性表达导致干细胞多能性和自我更新的减少(11)。

  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
  11. Ho, L. et al. (2009) Proc Natl Acad Sci U S A 106, 5181-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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