Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMARCC2/BAF170 Antibody #8829

No. Size Price
8829S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8829 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 162, 170 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig, Horse,

Specificity / Sensitivity

SMARCC2/BAF170 Antibody recognizes endogenous levels of total SMARCC2/BAF170 protein (both isoforms 1 and 2).

SMARCC2/BAF170 Antibody能够检测内源性SMARCC1/BAF155总蛋白(亚型1和2)。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human SMARCC2/BAF170 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源SMARCC2/BAF170蛋白Ala223残基周围序列相对应的肽段免疫动物而生产的。通过蛋白A和多肽亲和层析纯化抗体获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SMARCC2/BAF170 Antibody.

使用SMARCC2/BAF170 Antibody,免疫印迹(Western blot)分析不同细胞中SMARCC2/BAF170蛋白水平。

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors such as nuclear receptors, p53, Rb, BRCA1, and MyoD facilitate recruitment of the complex to target genes to regulate gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

SMARCC2/BAF170 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by Brg1 in vitro (10). While SMARCC2/BAF170 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2/BAF170 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2/BAF170 leads to loss of stem cell pluripotency and self renewal (11).

ATP依赖的染色质修饰复合物在调节细胞核进程中起着重要的作用,例如转录、DNA复制和修复(1,2)。SWI/SNF染色质修饰复合物是有超过10个亚单位组成,并且包含BRM或BRG1的一个单独分子,这两个蛋白作为ATPase催化亚单位。ATPase亚单位的激活干扰组蛋白-DNA相互作用,并且改变了染色质重要调节元件的接近能力。另外中心的和附加的亚单位起到支撑作用去维持稳定性,并且提供了界面为不同转录因子和染色质相互作用(2-5)。SWI/SNF亚单位和转录因子例如细胞核受体、p53、Rb、BRCA1、MyoD之间的相互作用有助于复合物招募到靶基因去调节基因激活、细胞生长、细胞周期和分化进程(1,6-9)。

SMARCC2/BAF170是SWI/SNF复合物的中心亚单位之一,在体内该蛋白通过Brg1进行高效的核小体修饰是必须的(10)。在非多能干细胞中SMARCC2/BAF170已经显示是SWI/SNF复合物的部分,而在多能胚胎肝 (ES)细胞中是缺少的。SMARCC2/BAF170蛋白表达已经显示在神经元/神经祖细胞中是上调的,这些细胞是来自含视黄酸的小鼠ES细胞分化的,并且SMARCC2/BAF170蛋白外源性表达导致干细胞多能性和自我更新的减少(11)。

  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
  11. Ho, L. et al. (2009) Proc Natl Acad Sci U S A 106, 5181-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications