Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828

sc-11769   smad   smad2   smad23   smad3  

No. Size Price
8828S 100 µl ( 10 western blots ) ¥3,960.00 现货查询 购买询价
8828 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 52, 60 Rabbit IgG
F 1:800
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. This antibody also recognizes endogenous levels of Smad3 protein when phosphorylated Ser422 only or at both Ser423 and Ser425.

Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4)Rabbit mAb兔单抗可以识别Ser465和Ser467磷酸化的内源性的总Smad2蛋白。抗体可以识别仅Ser422被磷酸化或Ser423和Ser425同时被磷酸化的Smad3蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser465/467 of human Smad2 protein.




Confocal immunofluorescent analysis of HaCaT cells, serum-starved (left), treated with hTGF-β3 #8425 (100 ng/ml, 30 min; center), or treated with hTGF-β3 and SB 431542 (10 μg/mL, 1 hr; right), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (green) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8693 (red).

血清饥饿(左)的HaCaT细胞,使用hTGF-β3 #8425 (100 ng/ml, 30 min; 中),或使用hTGF-β3和SB 431542 (10 μg/mL, 1 hr;右)处理,使用Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4)Rabbit mAb(绿色)和COX IV (3E11)Rabbit mAb(偶联Alexa Fluor® 555偶联)#8693(红色)进行激光共聚焦免疫荧光分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT-1080 cells, untreated (blue), treated with hTGF-β3 #8425 (green), or treated with hTGF-β3 and SB 431542 (red), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb.

未处理(蓝色),用hTGF-β3 #8425(绿色)处理或hTGF-β3和SB 431542(红色)处理的HT-1080细胞,使用Phospho-Smad2 (Ser465/467)/Smad3(Ser423/425) (D27F4)Rabbit mAb兔单抗进行流式分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from HaCaT cells, untreated (-) or treated with hTGF-β3 #8425 (+) in the absence or presence of the TGFR inhibitor SB 431542, using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (upper) or Smad2/3 (D7G7) XP® Rabbit mAb #8685 (lower).

未处理(-)或在TGFR 抑制剂 SB 431542存在或不存在情况下处理hTGF-β3 #8425(+)HaCaT细胞,使用Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4)Rabbit mAb(上)或Smad2/3 (D7G7) XP® Rabbit mAb #8685(下)对细胞提取物进行western blot分析。


Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

Smad信号转导分子家族成员是胞内通路中TGF-β信号从细胞表面传送到细胞核的重要组成部分。Smad 可被分为三类:受体调控型Smad(简称R-Smad),主要包括Smad1,2,3,5和8;共同介导型Smad(co-Smad)包括Smad4;还有拮抗型或称抑制型Smad(I-Smads),包括Smad6和Smad7[1-5]。激活的I型受体与特异的R-Smad有关,并能在R-Smad的保守的C端SSXS基序处对其磷酸化。磷酸化的R-Smad与受体分离,再与co-Smad(Smad4)形成异侧复合物,使复合物迁移入核。一旦进入核内,Smad可以各种DNA绑定蛋白为目标调控转录反应。[6-8]

  1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
  2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
  3. Derynck, R. et al. (1998) Cell 95, 737-40.
  4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
  5. Whitman, M. (1998) Genes Dev 12, 2445-62.
  6. Wu, G. et al. (2000) Science 287, 92-7.
  7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
  8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

Application References

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