Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb #8826

No. Size Price
8826S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8826 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 91 Rabbit IgG
IP 1:100
IHC-P 1:800
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Bovine,

Specificity / Sensitivity

Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Ser727.

Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb兔单抗仅能够检测当Ser727发生磷酸化时的内源性Stat1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser727 of human Stat1 protein.

此单克隆抗体通过合成肽免疫动物制备,这种合成肽是人源Stat1蛋白Ser727周围的肽段。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma, control (left) or λ phosphatase-treated (right) using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb. 对石蜡包埋的人淋巴瘤(对照)(左图)或 λ磷酸化处理的(右图)进行免疫组化分析,使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 10 μl of Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HT-1080细胞中的交联染色质,细胞通过IFN-γ (50 ng/ml)处理30分钟或者使用 10 μl Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb 或 2 μl of Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, 和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀得到的DNA总量与投入的核酸总量相当。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, untreated (-) or UV-treated (2 hr recovery; +), using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb. Western blot分析多种细胞系的细胞提取物,未处理(-)或UV处理(2 hr 复原; +),使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from UV-treated HeLa cells, untreated (-) or CIP and λ phosphatase-treated (+), using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (upper) or Stat1 (42H3) Rabbit mAb #9175 (lower). Western blot分析UV处理的HeLa细胞的细胞提取物,未处理(-)或CIP和 λ磷酸化处理(+),使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (上图) 或 Stat1 (42H3) Rabbit mAb #9175 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 30 min; +), using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (upper) or Stat1 (42H3) Rabbit mAb #9175 (lower). Western blot分析HeLa细胞的细胞提取物,未处理(-)或 Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 30 分钟; +)处理, 使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (上图) 或 Stat1 (42H3) Rabbit mAb #9175 (下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb. 对石蜡包埋的人乳腺癌组织进行免疫组化分析,使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb. 对石蜡包埋的卵巢癌组织进行免疫组化分析,使用抗体是Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb。

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Stat1转录因子可以被一系列配体所激活(1), 其中对IFN-α和 IFN-γ的应答非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1形成二聚体, 入核后与DNA结合(4)。 Stat1 蛋白是以Stat1α (91 kDa) 和 剪接变体Stat1β (84 kDa)二聚体的形式存在。在很多细胞中,这两种形式都是通过IFN-α激活。但是只有 Stat1α是被 IFN-γ激活。Stat1这种不正常的激活方式存在于很多肿瘤(5)。除了酪氨酸磷酸化,Stat1 也能通过p38有丝分裂激活蛋白激酶依赖(MAPK)依赖的通路在Ser727位点发生磷酸化而被激活,从而对IFN-α和其它的细胞应激产生应答(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

  1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
  2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
  3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
  4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
  5. Frank, D.A. (1999) Mol Med 5, 432-56.
  6. Wen, Z. et al. (1995) Cell 82, 241-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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