Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Synapsin-1 (Ser605) (D4B9I) Rabbit mAb #88246

No. Size Price
88246S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
88246 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75-90 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Synapsin-1 (Ser605) (D4B9I) Rabbit mAb recognizes endogenous levels of Synapsin-1 protein only when phosphorylated at Ser605 (corresponds to Ser603 in rat).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser605 of human Synapsin-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, mouse lung, and rat brain using Phospho-Synapsin-1 (Ser605) (D4B9I) Rabbit mAb (upper) and and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of mouse brain lysate, untreated (-) or λ phosphatase-treated (+), using Phospho-Synapsin-1 (Ser605) (D4B9I) Rabbit mAb (upper) and and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).

  1. Greengard, P. (1987) Mol Neurobiol 1, 81-119.
  2. Hosaka, M. et al. (1999) Neuron 24, 377-87.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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