Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) #8822


No. Size Price
8822S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
8822T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
8822 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 29-42 Rabbit IgG
IP 1:100
F 1:200
IF-IC 1:1000
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse nanog protein. This antibody is expected to recognize nanog 1a and 1b isoforms. This antibody does not cross-react with human nanog.

Nanog (D2A3) XP® Rabbit mAb兔单抗(小鼠特异)识别内源性的总的小鼠nanog蛋白。预测此抗体识别nanog的异构体 1a 和1b,而与人源nanog没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from F9 and murine iPS cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific).



Confocal immunofluorescent analysis of F9 (left) and NIH/3T3 (right) cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 F9 cells and either 5 μl of Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 (blue) and F9 (green) cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific).


Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

Nanog是一个含有同源结构域的转录因子对于维持胚胎干细胞的多潜能性和自我更新非常重要(1)。Nanog的表达由包括Sox2和关键多潜能性调控因子Oct-4在内的调控网络调控(1)。近来对于体细胞重编程的研究进展主要通过整合nanog, Oct-4, Sox2, KLF4, c-Myc, 和 LIN28等转录因子的病毒表达来实现 (2,3).

  1. Kim, J. et al. (2008) Cell 132, 1049-61.
  2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
  3. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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