Cell Signaling Technology

Product Pathways - Protein Stability

VCIP135 Antibody #88153

DUBA3   DUBs   VCIP1   VCIP135   VCPIP1  

No. Size Price
88153S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
88153 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 140 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

VCIP135 Antibody recognizes endogenous levels of total VCIP135 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1035 of human VCIP135 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VCIP135 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human VCIP135 protein (hVCIP135; +), using VCIP135 Antibody (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).



Immunoprecipitation of VCIP135 from 293T cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitation with Normal Rabbit IgG #2729, and lane 3 is immunoprecipitation with VCIP135 Antibody. Western blot analysis was performed using VCIP135 Antibody.


Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, and JAMM. The valosin-containing protein p97/p47 complex-interacting protein 1 (VCIP135, VCPIP1) is a deubiquitinating enzyme that belongs to the A20-like subfamily of ovarian tumor (OTU) DUBs (3). VCIP135 serves as a cofactor for the p97/p47 complex in regulating Golgi membrane fusion and reassembly at the end of mitosis (4-6). Research studies suggest that the phosphorylation status of VCIP135 provides a mechanism to fine-tune the kinetics of Golgi disassembly and reassembly during the cell cycle. For example, these studies demonstrate that VCIP135 undergoes phosphorylation early in mitosis, which blocks its association with the Golgi membrane and p97/VCP, thus inhibiting p97/VCP-mediated Golgi membrane fusion (7,8).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Mevissen, T.E. et al. (2013) Cell 154, 169-84.
  4. Wang, Y. et al. (2004) J Cell Biol 164, 973-8.
  5. Uchiyama, K. et al. (2002) J Cell Biol 159, 855-66.
  6. Totsukawa, G. et al. (2011) EMBO J 30, 3581-93.
  7. Zhang, X. et al. (2014) J Cell Sci 127, 172-81.
  8. Totsukawa, G. et al. (2013) Biochem Biophys Res Commun 433, 237-42.

Application References

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