Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb #8808

No. Size Price
8808S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8808 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 175 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb detects endogenous levels of EGFR protein only when phosphorylated at Thr669. While the literature refers to this residue as Thr669, it is Thr693 of human EGFR (UniProt sequence P00533) and corresponds to Thr695 of mouse EGFR or Thr694 of rat EGFR.

只有当Thr669磷酸化时,磷酸化EGF Receptor (Thr669) (D2F1) 兔单抗能够检测内源性水平的EGFR蛋白。当文献提到残基是Thr669,即是人EGFR(UniProt序列P00533) Thr693和对应的小鼠EGFR的Thr695或大鼠EGFR的Thr694.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr693 of human EGFR protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells serum starved overnight, and left untreated (-) or treated (+) with EGF (100 ng/ml, 15 min), using Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb (upper) and EGF Receptor (D38B1) XP® Rabbit mAb #4267 (lower).Western blot方法检测血清饥饿过夜处理的A-431细胞提取物,剩余细胞不处理(-)或用EGF (100 ng/ml, 15 min)处理(+),使用的抗体为Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb (上图)和EGF Receptor (D38B1) XP® Rabbit mAb #4267 (下图).


The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

表皮生长因子(EGF)受体是一个属于HER/ErbB蛋白家族的跨膜酪氨酸激酶。配体的结合导致了受体的二聚化,自磷酸化,下游信号分子的激活,内化以及溶酶体降解 (1,2)。表皮生长因子受体(EGFR)在激酶域Tyr845位点的磷酸化与稳定激活回路有关,并且维持了酶的激活状态,为底物蛋白提供了可结合的表位(3,4)。c-Src与 EGFR 在 Tyr845位点的磷酸化有关(5)。PLCγ的SH2结构域结合到磷酸化的Tyr992位点,将引起PLCγ介导的下游信号通路的激活(6)。EGFR Tyr1045位点的磷酸化则为c-Cbl生成一个主要的停靠位点,这个衔接蛋白将导致受体的泛素化以及EGFR活化后的降解(7,8)。GRB2衔接蛋白则结合到活化后的EGFR磷酸化的Tyr1068位点(9)。一对磷酸化EGFR残基(Tyr1148 and Tyr1173)为Shc支架蛋白提供了停靠点,这两个位点都与MAPK激酶通路的激活有关(2)。EGFR在特定的丝氨酸和苏氨酸残基上的磷酸化减弱了EGFR激酶的活性。EGFR羧基末端残基Ser1046 和 Ser1047被CaM 激酶II磷酸化,这两个丝氨酸任何一个突变都将导致EGFR酪氨酸自身磷酸化的上调(10)。

Thr669 (equivalent to Thr693 of human EGFR) is phosphorylated by p38 MAP kinase following EGF stimulation (11). Phosphorylation of EGFR at Thr669 may be involved in regulation of ligand induced receptor internalization through interaction with specific downstream EGFR tyrosine kinase substrates (11).

Thr669(等同于人EGFR的Thr693)在EGF刺激后,由p38 MAPK激酶磷酸化(11)。EGFR中Thr669的磷酸化可能通过与特定下游EGFR酪氨酸激酶底物的相互作用,参与调节配体诱导受体内在化(11)。

  1. Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
  2. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
  3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
  4. Hubbard, S.R. et al. (1994) Nature 372, 746-54.
  5. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
  6. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
  7. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
  8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
  9. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
  10. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
  11. Winograd-Katz, S.E. and Levitzki, A. (2006) Oncogene 25, 7381-90.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :