Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) #8785

No. Size Price
8785S 100 µl ( 20 immunoprecipitations ) ¥3,100.00 现货查询 购买询价
8785 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:100 Mouse, Endogenous Rabbit IgG
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) immunoprecipitates endogenous levels of total mouse nanog protein. This antibody does not cross-react with human nanog protein.Nanog (D1G10) Rabbit mAb兔单抗 (小鼠特异; ChIP 级别)可免疫沉淀小鼠内源的总nanog蛋白,而与人源nanog蛋白没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与小鼠nanog蛋白邻近氨基末端的氨基酸序列一致。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 F9 cells and either 5 μl of Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

Nanog是一个含有同源结构域的转录因子,对于维持胚胎干细胞的多潜能性和自我更新非常重要(1)。Nanog的表达由包括Sox2和关键多潜能性调控因子Oct-4在内的调控网络调控(1)。近来对于体细胞重编程的研究进展主要通过整合包含nanog, Oct-4, Sox2, KLF4, c-Myc和 LIN28等转录因子病毒表达来实现 (2,3).

  1. Kim, J. et al. (2008) Cell 132, 1049-61.
  2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
  3. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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