Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3α (D1A5) XP® Rabbit mAb #8768

No. Size Price
8768S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
8768T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
8768 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 86 Rabbit IgG
IP 1:50
IHC-P 1:500
IF-IC 1:200
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Pig,

Specificity / Sensitivity

Stat3α (D1A5) XP® Rabbit mAb recognizes endogenous levels of total Stat3α protein. This antibody does not cross-react with Stat3β.

Stat3α (D1A5) XP® Rabbit mAb兔单抗能够检测内源性Stat3α总蛋白水平。这种抗体不与Stat3β发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Stat3α protein.




Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/mL, 30 min; right) using Stat3α (D1A5) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). 共聚焦分析HeLa细胞,血清饥饿(左图)或Human Interferon-α1 (hIFN-α1) #8927 (100 ng/mL, 30 分钟; 右图)处理,使用抗体是Stat3α (D1A5) XP® Rabbit mAb (绿色) 和 β-Actin (8H10D10) Mouse mAb #3700 (红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Stat3α (D1A5) XP® Rabbit mAb (top), Stat3 (124H6) Mouse mAb #9139 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot分析多种细胞系的细胞提取物,使用抗体是Stat3α (D1A5) XP® Rabbit mAb (上图), Stat3 (124H6) Mouse mAb #9139 (中图),或β-Actin (D6A8) Rabbit mAb #8457 (下图)。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min), and either 5 μl of Stat3α (D1A5) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀法检测4 x 106 HepG2细胞中的交联染色质,细胞通过饥饿过夜或者使用Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 分钟) 或5 μl 的 Stat3α (D1A5) XP® Rabbit mAb或2 μl of Normal Rabbit IgG #2729处理。使用试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。丰富的DNA通过使用human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663,和SimpleChIP® Human α Satellite Repeat Primers #4486引物进行Real-Time PCR来量化。每个样品中免疫沉淀得到的DNA总量与投入的核酸总量相当。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Stat3α (D1A5) XP® Rabbit mAb in the presence of control peptide (left) or antige-specific peptide (right). 对石蜡包埋的人结肠癌组织进行免疫组化分析,使用抗体是Stat3α (D1A5) XP® Rabbit mAb含有对照抗原肽(左图) 或者特异性抗原肽(右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or PC-3 (right), using Stat3α (D1A5) XP® Rabbit mAb. 对石蜡包埋的细胞颗粒,HeLa(左图)或PC-3(右图)进行免疫组化分析,使用抗体是Stat3α (D1A5) XP® Rabbit mAb。


The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路中重要的信号分子(1),并且对于小鼠胎儿发育是必须的(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性肿瘤的可能(5) ,而且也具有抗凋亡活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核后与DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Stat3 Ser727位点的磷酸化的调控(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型、配体方向或者细胞发育的成熟阶段(10)。值得注意的是,Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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