Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IKKε (Ser172) (D1B7) Rabbit mAb #8766

No. Size Price
8766S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8766 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Dog,

Specificity / Sensitivity

Phospho-IKKε (Ser172) (D1B7) Rabbit mAb recognizes endogenous levels of IKKε protein only when phosphorylated at Ser172.

Phospho-IKKε (Ser172) (D1B7) Rabbit mAb兔单抗仅仅能够检测内源性的Ser172位点发生磷酸化的IKKε蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human IKKε protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, indicated times), using Phospho-IKKε (Ser172) (D1B7) Rabbit mAb (upper) or IKKε (D20G4) Rabbit mAb #2905 (lower).Western blot分析THP-1细胞的细胞提取物,通过TPA #4174 (80 nM, 过夜)分离,紧接着使用LPS (1 μg/ml,指示时间)处理,使用抗体是Phospho-IKKε (Ser172) (D1B7) Rabbit mAb (上图) 或 IKKε (D20G4) Rabbit mAb #2905 (下图)。


The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分因子通过一个经典的信号通路,即磷酸化诱导、蛋白酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶(IKK)复合体,此复合体催化的反应中有三个紧密相关的IKK亚基参与完成,IKKα and IKKβ 作为激酶的催化单元,IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化:IKKβ活性环中的 Ser177和Ser181位点(IKKα中的ser176和ser180 位点),这些特异性的位点的磷酸化导致蛋白构象的变化从而激活激酶(10-13)。

Recently, two homologs of IKKα and IKKβ have been described, called IKKε (also known as IKK-i) and TBK1 (also known as T2K or NAK), and activation of either of these kinases results in NF-κB activation. IKKε contains the kinase domain in its amino terminus, which shares 30% identity to that of IKKα or IKKβ. IKKε is expressed mainly in immune cells, and may play a special role in the immune response (14-18). IKKε and TBK1 kinase capabilities are activated by phosphorylation at Ser172 within their activation loops (19). IRF-3, a substrate for IKKε and TBK1, plays a critical role in innate immune responses (20).

最近,IKKα和IKKβ的两个同源蛋白得到了鉴定,分别是IKKε (也叫IKK-i)和TBK-1 (也叫T2K 或 NAK), 激活这些激酶中的任何一个都能导致NF-κB的激活。IKKε在N-末端包含一个与IKKα或IKKβ有30%相似度的激酶结构域。IKKε主要在免疫细胞中表达,并在免疫应答中发挥了特殊作用(14-18)。IKKε 和 TBK1能够通过活化环内的Ser172位点的磷酸化而被激活(19)。IRF-3,是IKKε 和 TBK1的底物,在先天性免疫应答中发挥着重要的作用(20)。

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Application References

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