Product Pathways - Protein Stability
UBR5 Antibody #8755
|8755S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|8755||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Species predicted to react based on 100% sequence homology: Dog, Pig,
Specificity / Sensitivity
UBR5 Antibody recognizes endogenous levels of total UBR5 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBR5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Human E3 identified by differential display (UBR5/EDD) is a 300 kDa HECT domain-containing ubiquitin E3 ligase that is a component of the N-end rule pathway and recognizes proteins bearing specific destabilizing N-terminal residues, leading to their ubiquitination and subsequent degradation (1). UBR5 represents an ortholog of HYD, the Drosophila hyperplastic discs tumor suppressor gene product. UBR5 mRNA is expressed in many tissues and is frequently overexpressed in breast and ovarian cancers, suggesting a possible role in tumor development (2-4). UBR5 contains three putative nuclear localization signals, an N-terminal UBA domain (mediates interaction of UBR5 with ubiquitin), a central UBR1-like zinc finger motif (mediates substrate recognition), a PABC domain (peptide domain found in the poly(A)-binding protein PABP), and a C-terminal HECT ubiquitination domain (2,5-8). UBR5 has been shown to bind to and ubiquitinate the topoisomerase II beta-binding protein TopBP1, and to bind to and potentiate progesterone receptor-mediated transcriptional transactivation (9,10). More recently, it has been reported that UBR5 binds to the DNA damage checkpoint kinase Chk2 and acts upstream of Chk2 (11), supporting the notion that UBR5 is involved in pathways regulating the DNA damage response. Furthermore, it has been demonstrated that downregulation of PABP results in UBR5-mediated degradation of the PABP-associated protein Paip2, which interferes with protein translation by displacing PABP from mRNA (12). This indicates that UBR5 is involved in the regulation of PABP activity and, thus, in controlling translation efficiency.
- Sriram, S.M. et al. (2011) Nat Rev Mol Cell Biol 12, 735-47.
- Callaghan, M.J. et al. (1998) Oncogene 17, 3479-91.
- Tasaki, T. et al. (2005) Mol Cell Biol 25, 7120-36.
- Clancy, J.L. et al. (2003) Oncogene 22, 5070-81.
- O'Brien, P.M. et al. (2008) Br J Cancer 98, 1085-93.
- Munoz, M.A. et al. (2007) Cell Cycle 6, 3070-7.
- Henderson, M.J. et al. (2006) J Biol Chem 281, 39990-40000.
- Honda, Y. et al. (2002) J Biol Chem 277, 3599-605.
- Gudjonsson, T. et al. (2012) Cell 150, 697-709.
- Rutz, S. et al. (2015) Nature 518, 417-21.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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