Cell Signaling Technology

Product Pathways - DNA Damage

XPB (2C6) Mouse mAb #8746

sc-293  

No. Size Price
8746S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8746T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
8746 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 89 Mouse IgG2a
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

XPB (2C6) Mouse mAb recognizes endogenous levels of total XPB protein.XPB (2C6) Mouse mAb能够检测内源性XPB总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human XPB protein.该单克隆抗体是由重组的人源的XPB蛋白羧基末端肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using XPB (2C6) Mouse mAb.Western blot 方法检测不同细胞提取物,使用的抗体为 XPB (2C6) Mouse mAb。

Background

XPB and XPD are ATPase/helicase subunits of the TFIIH complex that are involved in nucleotide excision repair (NER) to remove lesions and photoproducts generated by UV light (1). XPB and XPD are 3’-5’ and 5’-3’ DNA helicases, respectively, that play a role in opening of the DNA damage site to facilitate repair (2,3). XPB and XPD both play an important role in maintaining genomic stability, and mutations of these proteins cause Xeroderma Pigmentosum (XP) and Trichothiodystrophy (TTD). XP patients have abnormalities in skin pigmentation and are highly susceptible to skin cancers, while TTD patients exhibit symptoms such as brittle hair, neurological abnormalities, and mild photosensitivity (4). In addition to their role in NER, XPB and XPD are involved in transcription initiation as part of the TFIIH core complex (5). The helicase activity of XPB unwinds DNA around the transcription start site to facilitate RNA polymerase II promoter clearance and initiation of transcription (6). XPD plays a structural role linking core TFIIH components with the cdk-activating kinase (CAK) complex that phosphorylates the C-terminus of the largest subunit of RNA polymerase II, leading to transcription initiation (7).XPB和XPD是TFIIH复合体的ATP酶/解旋酶亚基,参与核苷酸切除修复(NER)以清除损伤和紫外产生的磷酸化产物(1)。XPB和XPD分别是3'-5'和5'-3'DNA解旋酶,在打开DNA损伤部位以便进行维修复的过程中发挥作用(2,3)。 XPB和XPD在维护基因组的稳定性以及这些蛋白质的基因突变造成着色性干皮病(XP)和毛发硫营养不良((TTD)的过程中发挥了重要的作用。XP病人有异常的皮肤色素沉着,并具有很高的皮肤癌易感性,而TTD患者表现出其它的症状,如发脆,神经系统异常,轻度的光敏性(4)。除了它们在NER中的作用,XPB和XPD作为TFIIH核心复合物的一部分参与转录起始(5)。XPB的解旋酶活性能够在转录起始位点周围解旋DNA,促进RNA聚合酶II启动子清除和转录起始(6)。 XPD通过连接核心TFIIH组件与cdk活化激酶(CAK)复合物来发挥结构功能,磷酸化RNA聚合酶II最大亚基的C-末端,导致转录起始(7)。

  1. Oksenych, V. and Coin, F. (2010) Cell Cycle 9, 90-6.
  2. Evans, E. et al. (1997) EMBO J 16, 6559-73.
  3. Riedl, T. et al. (2003) EMBO J 22, 5293-303.
  4. Lehmann, A.R. (2003) Biochimie 85, 1101-11.
  5. Drapkin, R. et al. (1994) Nature 368, 769-72.
  6. Holstege, F.C. et al. (1996) EMBO J 15, 1666-77.
  7. Rossignol, M. et al. (1997) EMBO J 16, 1628-37.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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