Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-CK2 Substrate [(pS/pT)DXE] MultiMab™ Rabbit mAb mix #8738

CK2 substrate   Motif antibody  

No. Size Price
8738S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8738 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb recognizes endogenous proteins containing a pS/pTDXE motif, which is a CK2 phosphorylation consensus sequence. This antibody is a useful tool to study CK2 substrates.

Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb兔单抗能够检测内源性的含有CK2 磷酸化共有序列pS/pTDXE 基序的蛋白。该抗体是研究CK2底物的有力工具。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the T*DXE motif.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902 (10 nM, 30 min) or Staurosporine #9953 (1 μM, 3 hr), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb.

Western blot方法分别检测未处理或经Calyculin A #9902 (10 nM)处理,30 min 或 Staurosporine #9953 (1 μM)处理3 hr的HeLa细胞提取物,使用的抗体为Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CK2α siRNA I #6389 (+), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb (upper), CK2α Antibody #2656 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Western blot方法分别检测经SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® CK2α siRNA I #6389 (+)转染的HeLa细胞提取物,使用的抗体为Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb (上图), CK2α Antibody #2656 (中图), 和 GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。


Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric Ser/Thr protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer, and other diseases (1-5). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated Ser or Thr residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position followed by the residue at the +1 position (6).

Casein Kinase II (CK2)是一个高度保守、广泛表达,具有组成型活性的四聚体Ser/Thr 蛋白激酶,具有多种参与细胞周期进程、凋亡、转录、炎症以及DNA损伤应激等一系列细胞进程调控的底物。研究发现,CK2与病毒感染、癌症及其他疾病相关(1-5)。CK2底物包含多种酸性残基(Asp和 Glu),位于磷酸化Ser或Thr残基的下游。CK2底物的共有序列是pS/pTD/EXD/E,最关键的是+3 位的氨基酸残基,之后是+1 位的氨基酸残基(6)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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