Cell Signaling Technology

Product Pathways - Protein Stability

USP8 Antibody #8728

ubiquitin   UBPy   USP8  

No. Size Price
8728S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8728T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
8728 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 130 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

USP8抗体识别内源性的USP8总蛋白。

Source / Purification

USP8多克隆抗体是通过合成与人USP8蛋白Pro320邻近氨基酸残基序列一致的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from various cell lines using USP8 Antibody. 使用USP8抗体对不同细胞裂解物进行免疫印迹分析的结果。 Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected (+) with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP8, using USP8 Antibody. 使用USP8抗体对转染对照载体(-)或转染带Myc/DDK标签的人USP8基因表达载体(+)的293T细胞提取物进行免疫印迹分析的结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP8 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected (+) with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP8, using USP8 Antibody.

Background

泛素酶(UBEs)催化蛋白质泛素化,去泛素化酶(DUB)作用相反(1,2)。共有5个亚家族的去泛素化酶被发现,包括USP, UCH, OTU, MJD和JAMM酶。去泛素化酶USP8/UBPy(泛素特异性蛋白酶8)是一种半胱氨酸蛋白酶,属于USP/UBP亚家族。有证据表明USP8是一个是细胞增殖和生存必不可少的生长调节酶(3,4)。事实上,USP8基因条件敲除的小鼠会导致受体酪氨酸激酶表达缺失,包括EGFR、ERB-B3和c-Met(4)。与这些结果一致,USP8失活导致由配体激活的表皮生长因子受体的泛素化增强(5,6)。此外,USP8 Ser680磷酸化会促进14-3-3的结合并导致其催化能力的失活,减少EGFR的去泛素化(7)。看来,USP8似乎通过结合ESCRT0复合物的组分,在保护EGFR并防止被溶酶体降解的早期内体分选系统中起着不可或缺的作用,(8,9)。

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Naviglio, S. et al. (1998) EMBO J 17, 3241-50.
  4. Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39.
  5. Alwan, H.A. and van Leeuwen, J.E. (2007) J Biol Chem 282, 1658-69.
  6. Mizuno, E. et al. (2005) Mol Biol Cell 16, 5163-74.
  7. Mizuno, E. et al. (2007) Exp Cell Res 313, 3624-34.
  8. Row, P.E. et al. (2006) J Biol Chem 281, 12618-24.
  9. Berlin, I. et al. (2010) J Biol Chem 285, 34909-21.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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