Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb #8722

No. Size Price
8722S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
8722T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
8722 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75 rodent, 80 human Rabbit IgG
F 1:200
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine,

Specificity / Sensitivity

Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb recognizes endogenous levels of MARCKS protein only when phosphorylated at Ser167 and Ser170. This antibody may also detect MARCKS mono-phosphorylated at Ser167.

只有当丝氨酸(167和170位)磷酸化时,Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb兔单抗识别内源性的总MARCKS蛋白。该抗体也可以检测单-磷酸化MARCKS丝氨酸(167位)。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 and Ser170 of human MARCKS protein.

该单克隆抗体通过用合成磷酸肽免疫动物制备,该合成肽与人MARCKS蛋白丝氨酸(167和170位)附近的残基一样。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; middle) or treated with TPA #4174 and λ phosphatase (right), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦荧光法检测血清饥饿处理的HeLa细胞 (左图),细胞用TPA #4174 (200 nM, 15 min; 中图) 或TPA #4174和λ 磷酸酶处理(右图),使用的抗体为 Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (绿色). 蓝色伪彩色为DNA荧光染料 (产品信息为DRAQ5® #4084).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, serum-starved overnight and then untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MARCKS (Ser167/Ser170) (D13E4) XP® Rabbit mAb.

Western blot方法检测多个细胞系的提取物,细胞血清饥饿过夜,随后不处理(-)或TPA #4174 (200 nM, 15 min; +)处理,使用的抗体为Phospho-MARCKS (Ser167/Ser170) (D13E4) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved SH-SY5Y and SK-N-MC cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (upper) and MARCKS (D88D11) XP® Rabbit mAb #5607 (lower).

Western blot方法检测血清饥饿处理的SH-SY5Y和SK-N-MC细胞的提取物,细胞不处理(-)或经TPA #4174 (200 nM, 15 min; +)处理,使用的抗体为Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb (上图)和MARCKS (D88D11) XP® Rabbit mAb #5607 (下图).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, serum starved (blue) or serum starved and treated with TPA #4174 (200nM, 15 min; green), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary Ab.

流式分析Hela细胞,血清饥饿(蓝色)或血清饥饿后使用TPA#4174处理(200nM,15分钟;绿色),使用Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414为二抗.

Background

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

豆寇酰化富含丙氨酸C-激酶底物(MARCKS)是一种重要的PKC底物,在多种细胞类型中表达。 MARCKS与细胞活力,细胞粘附,吞噬,膜运输和有丝分裂有关(1)。PKC响应生长因子和氧化应激磷酸化MARCKS的丝氨酸(159,163,167和170位),调节钙/钙调蛋白结合和MARCKS丝状(F)-肌动蛋白交联反应活性(2-4)。PKC磷酸化作用导致MARCKS从细胞膜到细胞质迁移(5)。

  1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
  2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
  3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
  4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
  5. Thelen, M. et al. (1991) Nature 351, 320-2.

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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