Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714

No. Size Price
8714S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
8714T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
8714 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit IgG
F 1:200
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.

Phospho-HS1 (Tyr397) 抗体只能检测内源的在Tyr397位点磷酸化的HS1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

单克隆抗体是通过合成鼠源对应的HS1 Tyr405位点周围的肽段来免疫动物而获得。此位点对应人HS1蛋白的Tyr397位点。

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, serum starved for 12 hours, then treated with anti-IgM antibody or λ phosphatase, using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (upper), or HS1 Antibody (Human Specific) #4503 (lower).Western免疫印迹。用Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (上图) 或 HS1 Antibody (人特异性) #4503 (下图) 研究血清饥饿12小时并经anti-IgM antibody 或 λ phosphatase 处理的Ramos 细胞的细胞提取液。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Ramos cells, untreated (left) or treated with IgM (12 μg/mL, 10 min, right) using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析(左图) 或 经IgM (12 μg/mL, 10 min, 右图)处理的Ramos细胞,所用抗体为Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (绿色)。Blue pseudocolor= DRAQ5® #4084 (DNA荧光染料)

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or IgM-treated (green), using Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb.流式细胞仪分析未经处理(蓝色) 或经IgM处理(绿色)的Ramos细胞,所用抗体为Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb。

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

HS1 (HCLS1, LckBP1, p75)是蛋白激酶的底物,它只在具有造血功能的组织和细胞中表达(1,2)。HS1 包含了四个皮肌动蛋白重复序列和一个SH3 域(2)。在受到免疫受体的激活后此胞内蛋白被磷酸化,这就促进了HS1被招募到免疫突触(3-5)。磷酸化HS1是调控肌动蛋白动力不可缺少的,同时也为其他的信号分子如Vav1和 PLCγ1 提供了结合位点(6)。HS1也在血小板激活中发挥了重要的作用(7)。

HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).

在免疫受体刺激和凝血酶介导的血小板刺激下HS1能够快速的被Syk和/或 Lyn激酶在Tyr397位点磷酸化. 此磷酸化是细胞骨架重排和信号复合体形成过程中重要的一步(6-10)。

  1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
  2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
  4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
  5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
  6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
  7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
  8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
  9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
  10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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