Cell Signaling Technology

Product Pathways - Metabolism

CIAO1 (D4E2U) Rabbit mAb #87027

No. Size Price
87027S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
87027 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 41 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CIAO1 (D4E2U) Rabbit mAb recognizes endogenous levels of total CIAO1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe292 of human CIAO1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CIAO1 (D4E2U) Rabbit mAb.

Background

Iron-sulfur (Fe-S) clusters (ISC) are cofactors for many proteins that display a wide range of biological functions, such as DNA maintenance, transcription, translation, cellular metabolism, electron transport, and oxidative phosphorylation (1). While structurally simple, the synthesis and insertion of ISC into Fe-S proteins are complex processes that involve many different proteins. The cytosolic iron-sulfur assembly component 1 (CIAO1) protein is a key component of the cytosolic ISC assembly machinery that incorporates ISC into cytoplasmic and nuclear Fe-S proteins in eukaryotic cells (1,2). CIAO1, along with MMS19, XPD, FAM96B, and ANT2, comprise a complex that localizes to the mitotic spindle during mitosis, which suggests a role in chromosome segregation (3-6). The CIAO1 protein interacts with Wilms' tumor suppressor protein (WT1) and may affect its transactivation activity (7).

  1. Wang, J. and Pantopoulos, K. (2011) Biochem J 434, 365-81.
  2. Stehling, O. et al. (2013) Cell Metab 18, 187-98.
  3. Ito, S. et al. (2010) Mol Cell 39, 632-40.
  4. Gari, K. et al. (2012) Science 337, 243-5.
  5. Stehling, O. et al. (2012) Science 337, 195-9.
  6. Seki, M. et al. (2013) J Biol Chem 288, 16680-9.
  7. Johnstone, R.W. et al. (1998) J Biol Chem 273, 10880-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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