Cell Signaling Technology

Product Pathways - Metabolism

CIAO1 (D4E2U) Rabbit mAb #87027

No. Size Price
87027S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
87027 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 41 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

CIAO1 (D4E2U) Rabbit mAb recognizes endogenous levels of total CIAO1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe292 of human CIAO1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CIAO1 (D4E2U) Rabbit mAb.


Iron-sulfur (Fe-S) clusters (ISC) are cofactors for many proteins that display a wide range of biological functions, such as DNA maintenance, transcription, translation, cellular metabolism, electron transport, and oxidative phosphorylation (1). While structurally simple, the synthesis and insertion of ISC into Fe-S proteins are complex processes that involve many different proteins. The cytosolic iron-sulfur assembly component 1 (CIAO1) protein is a key component of the cytosolic ISC assembly machinery that incorporates ISC into cytoplasmic and nuclear Fe-S proteins in eukaryotic cells (1,2). CIAO1, along with MMS19, XPD, FAM96B, and ANT2, comprise a complex that localizes to the mitotic spindle during mitosis, which suggests a role in chromosome segregation (3-6). The CIAO1 protein interacts with Wilms' tumor suppressor protein (WT1) and may affect its transactivation activity (7).

  1. Wang, J. and Pantopoulos, K. (2011) Biochem J 434, 365-81.
  2. Stehling, O. et al. (2013) Cell Metab 18, 187-98.
  3. Ito, S. et al. (2010) Mol Cell 39, 632-40.
  4. Gari, K. et al. (2012) Science 337, 243-5.
  5. Stehling, O. et al. (2012) Science 337, 195-9.
  6. Seki, M. et al. (2013) J Biol Chem 288, 16680-9.
  7. Johnstone, R.W. et al. (1998) J Biol Chem 273, 10880-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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